TIM22 translocation channel protein TIM22 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TIM22
Official Full Name: translocation channel protein TIM22
Primary source: SGD:S000002376
Locus tag: YDL217C
See related: AllianceGenome:SGD:S000002376; FungiDB:YDL217C; VEuPathDB:YDL217C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mitochondrion targeting sequence binding activity; protein transmembrane transporter activity; and structural molecule activity. Involved in protein insertion into mitochondrial inner membrane. Located in mitochondrion. Part of TIM22 mitochondrial import inner membrane insertion complex. Is active in mitochondrial inner membrane. Used to study mitochondrial myopathy. Human ortholog(s) of this gene implicated in combined oxidative phosphorylation deficiency 43. Orthologous to human TIMM22 (translocase of inner mitochondrial membrane 22). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See TIM22 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (67983..68606, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Functional crosstalk between the TIM22 complex and YME1 machinery maintains mitochondrial proteostasis and integrity.
Title: Functional crosstalk between the TIM22 complex and YME1 machinery maintains mitochondrial proteostasis and integrity.
Conserved regions of budding yeast Tim22 have a role in structural organization of the carrier translocase.
Title: Conserved regions of budding yeast Tim22 have a role in structural organization of the carrier translocase.
reduced phosphatidylcholine levels differentially affect the TIM22 and TIM23 complexes in mitochondrial protein transport.
Title: Phosphatidylcholine Affects Inner Membrane Protein Translocases of Mitochondria.
We propose that Mia40 not only is responsible for disulfide bond formation, but also assists the Tim22 protein in its integration into the inner membrane of mitochondria.
Title: Mitochondrial protein import: Mia40 facilitates Tim22 translocation into the inner membrane of mitochondria.
TIM22 as a dynamic channel solely active in the presence of its cargo proteins
Title: Awaking TIM22, a dynamic ligand-gated channel for protein insertion in the mitochondrial inner membrane.
When Hot13p is overexpressed, it may function to remodel the small TIM complexes during import.
Title: The role of Hot13p and redox chemistry in the mitochondrial TIM22 import pathway.
Phosphate carrier (PiC) can be sorted to either the TIM22 pathway or the TIM23 pathway for its import into yeast mitochondria
Title: The phosphate carrier has an ability to be sorted to either the TIM22 pathway or the TIM23 pathway for its import into yeast mitochondria.
The Tim21 structure represents a new alpha/beta-mixed protein fold with two alpha-helices flanked by an extended eight-stranded beta-sheet. Here we suggest a mechanism for the TOM-TIM interaction.
Title: The Tim21 binding domain connects the preprotein translocases of both mitochondrial membranes.
the carboxyl-terminal module of dicarboxylate carrier possesses major targeting information to facilitate the direct binding of the carrier protein to TIM22
Title: The carboxyl-terminal third of the dicarboxylate carrier is crucial for productive association with the inner membrane twin-pore translocase.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables mitochondrion targeting sequence binding	IBA Inferred from Biological aspect of Ancestor more info
enables mitochondrion targeting sequence binding	IDA Inferred from Direct Assay more info	PubMed
enables protein transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein transmembrane transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables structural molecule activity	IDA Inferred from Direct Assay more info	PubMed
enables structural molecule activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in protein insertion into mitochondrial inner membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein insertion into mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
involved_in protein insertion into mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
involved_in protein insertion into mitochondrial inner membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of TIM22 mitochondrial import inner membrane insertion complex	IBA Inferred from Biological aspect of Ancestor more info
part_of TIM22 mitochondrial import inner membrane insertion complex	IDA Inferred from Direct Assay more info	PubMed
part_of TIM22 mitochondrial import inner membrane insertion complex	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: translocation channel protein TIM22

NP_010064.1

Essential core component of mitochondrial TIM22 complex; involved in insertion of polytopic proteins into inner membrane; forms channel through which proteins are imported; different regions of Tim22p have functionally diverse roles in recruiting partner subunits to core channel

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

67983..68606 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180277.1 → NP_010064.1 TPA: translocation channel protein TIM22 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010064.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRD7, Q12328

UniProtKB/TrEMBL

A6ZXB6, B3LH97, B5VF60, C7GSW0, C8Z6Q2, G2WBV0, N1P6G0

Conserved Domains (1) summary

COG5596
Location:1 → 197

TIM22; Mitochondrial import inner membrane translocase, subunit TIM22 [Posttranslational modification, protein turnover, chaperones]