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    UBC7 E2 ubiquitin-conjugating protein UBC7 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 855036, updated on 7-Jul-2024

    Summary

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    Gene symbol
    UBC7
    Gene description
    E2 ubiquitin-conjugating protein UBC7
    Primary source
    FungiDB:YMR022W
    Locus tag
    YMR022W
    See related
    SGD:S000004624; AllianceGenome:SGD:S000004624; VEuPathDB:YMR022W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    DER2; QRI8
    Summary
    Enables ubiquitin-protein transferase activity. Involved in chromatin assembly or disassembly; fungal-type cell wall organization; and ubiquitin-dependent ERAD pathway. Located in endoplasmic reticulum membrane. Part of Doa10p ubiquitin ligase complex and Hrd1p ubiquitin ligase ERAD-L complex. Orthologous to human UBE2G2 (ubiquitin conjugating enzyme E2 G2). [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    See UBC7 in Genome Data Viewer
    Location:
    chromosome: XIII
    Exon count:
    1
    Sequence:
    Chromosome: XIII; NC_001145.3 (318680..319177)

    Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene polyamine oxidase Neighboring gene Mac1p Neighboring gene Mss1p Neighboring gene tRNA-Ala

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001145.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Assays for dissecting the in vitro enzymatic activity of yeast Ubc7. Mashahreh B, et al. Methods Enzymol, 2019. PMID 30910030
    2. Ubiquitin-mediated regulation of 3-hydroxy-3-methylglutaryl-CoA reductase. Hampton RY, et al. Proc Natl Acad Sci U S A, 1997 Nov 25. PMID 9371780, Free PMC Article
    3. Resistance to cadmium mediated by ubiquitin-dependent proteolysis. Jungmann J, et al. Nature, 1993 Jan 28. PMID 8381213
    4. QRI8, a novel ubiquitin-conjugating enzyme in Saccharomyces cerevisiae. Vassal A, et al. Biochim Biophys Acta, 1992 Sep 24. PMID 1327148
    5. Hrd1 forms the retrotranslocation pore regulated by auto-ubiquitination and binding of misfolded proteins. Vasic V, et al. Nat Cell Biol, 2020 Mar. PMID 32094691

    See all (140) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Interaction of Ubc7 with the RING domains of its cognate E3 Ub-protein ligases stimulates the formation of isopeptide (amide) Ub-Ub linkages. This article provides protocols for dissecting the in vitro enzymatic activity of yeast Ubc7.
      Title: Assays for dissecting the in vitro enzymatic activity of yeast Ubc7.
    2. Sequential poly-ubiquitylation by specialized conjugating enzymes Ubc6 and Ubc7 expands the versatility of a quality control ubiquitin ligase Doa10.
      Title: Sequential Poly-ubiquitylation by Specialized Conjugating Enzymes Expands the Versatility of a Quality Control Ubiquitin Ligase.
    3. Cue1 positions Ubc7 and the distal acceptor ubiquitin for rapid polyubiquitination.
      Title: The CUE Domain of Cue1 Aligns Growing Ubiquitin Chains with Ubc7 for Rapid Elongation.
    4. Data indicate the crystal structure of the Ubc7p-binding region (U7BR) of Cue1p with Ubc7p.
      Title: A structurally unique E2-binding domain activates ubiquitination by the ERAD E2, Ubc7p, through multiple mechanisms.
    5. Data show that a discrete C-terminal Ubc7p binding region of Cue1p is required for ER-associated degradation and for Ubc7p-dependent ubiquitylation by Hrd1p in vitro.
      Title: A Ubc7p-binding domain in Cue1p activates ER-associated protein degradation.
    6. reveal a previously unobserved stimulation of Ubc7p E2 activity by Cue1p
      Title: Cue1p is an activator of Ubc7p E2 activity in vitro and in vivo.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin conjugating enzyme activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ubiquitin-protein transferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin-protein transferase activity ISS
    Inferred from Sequence or Structural Similarity
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in ERAD pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in ERAD pathway IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in ERAD pathway IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in ERAD pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in chromatin organization IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in fungal-type cell wall organization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in protein ubiquitination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in proteolysis involved in protein catabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to cadmium ion IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of Doa10p ubiquitin ligase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of Hrd1p ubiquitin ligase ERAD-L complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum HDA PubMed 
    is_active_in endoplasmic reticulum IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in endoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in endoplasmic reticulum membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in endoplasmic reticulum membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    E2 ubiquitin-conjugating protein UBC7
    NP_013735.1
    • Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation (ERAD) pathway and in the inner nuclear membrane-associated degradation (INMAD) pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001145.3 Reference assembly

      Range
      318680..319177
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182518.1 → NP_013735.1  TPA: E2 ubiquitin-conjugating protein UBC7 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013735.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VZJ6, Q02159
      UniProtKB/TrEMBL
      A0A6A5PRC4, A6ZM84, B3LLS3, C7GL97, C8ZEM9, G2WK73, N1P061
      Conserved Domains (1) summary
      COG5078
      Location:1 → 165
      COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q02159.1

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