ARP3 actin-related protein 3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ARP3
Gene description: actin-related protein 3
Primary source: FungiDB:YJR065C
Locus tag: YJR065C
See related: SGD:S000003826; AllianceGenome:SGD:S000003826; VEuPathDB:YJR065C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ACT4
Summary: Enables ATP binding activity. Involved in Arp2/3 complex-mediated actin nucleation and actin cortical patch organization. Located in actin cortical patch. Part of Arp2/3 protein complex. Human ortholog(s) of this gene implicated in opisthorchiasis. Orthologous to several human genes including ACTR3 (actin related protein 3) and ACTR3B (actin related protein 3B). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ARP3 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (557809..559158, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

These studies provide evidence that WASP (Las17) and WIP (Vrp1) play central roles in establishment of a robust multivalent Src homology 3 domain-proline-rich motif network in vivo, giving Actin-related protein 2/3 complex assembly onset at endocytic sites a switch-like behavior.
Title: Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo.
WASP displaces the autoinhibitory Arp3 C-terminal tail from a hydrophobic groove at Arp3's barbed end to destabilize the inactive state, providing a mechanism by which WASP stimulates the short-pitch conformation and activates the Arp2/3 complex.
Title: Role and structural mechanism of WASP-triggered conformational changes in branched actin filament nucleation by Arp2/3 complex.
Studies indicate that the WASP homologue Las17 is able to nucleate actin independently of Arp2/3 through the function of G-actin-binding activities in its polyproline region.
Title: WASP family proteins, more than Arp2/3 activators.
data does not support the simple hypothesis that the primary role for Arp2/3 regulators is to recruit and activate Arp2/3; rather data suggests that these regulators may be playing more subtle roles in establishing functional networks in vivo
Title: Molecular analysis of Arp2/3 complex activation in cells.
development of a direct tethering recruitment model for activation of Arp2/3 complex by Crn1
Title: Mechanism of a concentration-dependent switch between activation and inhibition of Arp2/3 complex by coronin.
These data identify Syp1 as a novel negative regulator of WASp-Arp2/3 complex that helps choreograph the precise timing of actin assembly during endocytosis.
Title: The F-BAR protein Syp1 negatively regulates WASp-Arp2/3 complex activity during endocytic patch formation.
Results identify an N-terminal fragment of Las17p able to interact with Vrp1p but not Arp2/3, and suggest that LAs17 interaction with Vrp1 is more important than interaction with Arp2/3 for polarized actin assembly and endocytosis in yeast.
Title: Las17p-Vrp1p but not Las17p-Arp2/3 interaction is important for actin patch polarization in yeast.
analysis of the key surfaces on p35/ARPC2 required for Arp2/3 complex-mediated actin assembly and cellular function
Title: Functional surfaces on the p35/ARPC2 subunit of Arp2/3 complex required for cell growth, actin nucleation, and endocytosis.
Act3p/Arp4 is a key regulator in the maintenance of cellular shape and nuclear organization
Title: The nuclear actin-related protein Act3p/Arp4 influences yeast cell shape and bulk chromatin organization.
investigated the ability of the yeast Arp2/3 complex (yArp2/3) to facilitate yeast actin polymerization in the presence and absence of the Arp2/3-activating factor Las17p WA
Title: Acceleration of yeast actin polymerization by yeast Arp2/3 complex does not require an Arp2/3-activating protein.

Submit: New GeneRIF Correction See all GeneRIFs (14)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables actin binding	IEA Inferred from Electronic Annotation more info
contributes_to actin filament binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in Arp2/3 complex-mediated actin nucleation	IBA Inferred from Biological aspect of Ancestor more info
involved_in Arp2/3 complex-mediated actin nucleation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cortical patch organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of Arp2/3 protein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Arp2/3 protein complex	IDA Inferred from Direct Assay more info	PubMed
is_active_in actin cortical patch	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cortical patch	IDA Inferred from Direct Assay more info	PubMed
located_in actin cortical patch	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in intracellular non-membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	HDA more info	PubMed

General protein information

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Preferred Names: actin-related protein 3

NP_012599.3

Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

557809..559158 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181723.3 → NP_012599.3 TPA: actin-related protein 3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012599.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWN6, P47117

UniProtKB/TrEMBL

A0A6A5PTL9, A6ZQ24, B3LQF3, C7GMD8, C8ZBL3, G2WH70, N1P145

Conserved Domains (2) summary

COG5277
Location:1 → 445

COG5277; Actin-related protein [Cytoskeleton]

PTZ00280
Location:5 → 446

PTZ00280; Actin-related protein 3; Provisional