REV3 DNA-directed DNA polymerase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: REV3
Official Full Name: DNA-directed DNA polymerase
Primary source: SGD:S000006088
Locus tag: YPL167C
See related: AllianceGenome:SGD:S000006088; FungiDB:YPL167C; VEuPathDB:YPL167C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: PSO1
Summary: Enables DNA-directed DNA polymerase activity. Involved in error-free translesion synthesis and error-prone translesion synthesis. Located in mitochondrion. Part of zeta DNA polymerase complex. Orthologous to human REV3L (REV3 like, DNA directed polymerase zeta catalytic subunit). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See REV3 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (232593..237107, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structure and mechanism of B-family DNA polymerase zeta specialized for translesion DNA synthesis.
Title: Structure and mechanism of B-family DNA polymerase ζ specialized for translesion DNA synthesis.
Bypass of DNA interstrand crosslinks by a Rev1-DNA polymerase zeta complex.
Title: Bypass of DNA interstrand crosslinks by a Rev1-DNA polymerase ζ complex.
The roles of REV3 in DNA replication and repair have been summarized. (Review)
Title: DNA polymerase ζ in DNA replication and repair.
Profiles of base-substitutions created by Polzeta & Poleta of Saccharomyces cerevisiae were analyzed. Poleta created deletions at the lesion in more than 50% of the TLS products, but the formation of the deletions was suppressed by the presence of Polzeta. Polzeta & Poleta cooperatively facilitated the TLS reaction over an abasic site in vitro, suggesting that these two polymerases can cooperate in efficient and high f...
Title: NGS-based analysis of base-substitution signatures created by yeast DNA polymerase eta and zeta on undamaged and abasic DNA templates in vitro.
This study also uncovered an important role for Rev3 and Rev7 (but not for Pol32) subunits of polymerase zeta in the survival of cells undergoing telomere losses and forming palindromes
Title: Telomere Dysfunction Triggers Palindrome Formation Independently of Double-Strand Break Repair Mechanisms.
A novel mutation affecting the catalytic subunit Rev3 and the role of Rev3 in the Pol32 regulation
Title: A novel variant of DNA polymerase ζ, Rev3ΔC, highlights differential regulation of Pol32 as a subunit of polymerase δ versus ζ in Saccharomyces cerevisiae.
Pol zeta, the enzyme responsible for the majority of damage-induced mutagenesis in eukaryotes, the two-subunit (Rev3-Rev7) form of yeast Pol zeta used in this study suggest that ribonucleotide incorporation into DNA during Pol zeta-mediated mutagenesis in vivo may be rare.
Title: Ribonucleotide incorporation by yeast DNA polymerase ζ.
study found both Pol zeta and Pol eta are necessary for efficient gap repair and that each functions independently of the other; these polymerases may be involved either in initiation of DNA synthesis from the invading end or in a gap-filling process that is required to complete recombination
Title: Roles of exonucleases and translesion synthesis DNA polymerases during mitotic gap repair in yeast.
in addition to extending aberrant termini created by other DNA polymerases, Pol zeta has the potential to be the sole DNA polymerase involved in translesion synthesis
Title: Lesion bypass by S. cerevisiae Pol ζ alone.
Results imply that about 30% of starvation-associated base substitutions and frameshifts arise by some unknown mechanism that does not involve Pol zeta or NHEJ.
Title: Evaluation of the roles of Pol zeta and NHEJ in starvation-associated spontaneous mutagenesis in the yeast Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 4 iron, 4 sulfur cluster binding	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-directed DNA polymerase activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA damage response	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in error-free translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in error-prone translesion synthesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in error-prone translesion synthesis	IDA Inferred from Direct Assay more info	PubMed
involved_in error-prone translesion synthesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in translesion synthesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in intracellular membrane-bounded organelle	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of zeta DNA polymerase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of zeta DNA polymerase complex	IDA Inferred from Direct Assay more info	PubMed
part_of zeta DNA polymerase complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-directed DNA polymerase

NP_015158.1

EC 2.7.7.7

Catalytic subunit of DNA polymerase zeta (pol zeta); involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; may be involved in meiosis; forms a complex with Rev7p, Pol31p and Pol32p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

232593..237107 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183981.1 → NP_015158.1 TPA: DNA-directed DNA polymerase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015158.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3K1, P14284

UniProtKB/TrEMBL

G2WP15, N1NVF6

Conserved Domains (4) summary

cd05534
Location:919 → 1366

POLBc_zeta; DNA polymerase type-B zeta subfamily catalytic domain. DNA polymerase (Pol) zeta is a member of the eukaryotic B-family of DNA polymerases and distantly related to DNA Pol delta. Pol zeta plays a major role in translesion replication and the production ...

COG0417
Location:398 → 1376

PolB; DNA polymerase elongation subunit (family B) [Replication, recombination and repair]

PTZ00166
Location:12 → 221

PTZ00166; DNA polymerase delta catalytic subunit; Provisional

pfam14260
Location:1398 → 1479

zf-C4pol; C4-type zinc-finger of DNA polymerase delta