RPA49 DNA-directed RNA polymerase I subunit RPA49 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: RPA49
Gene description: DNA-directed RNA polymerase I subunit RPA49
Primary source: FungiDB:YNL248C
Locus tag: YNL248C
See related: SGD:S000005192; AllianceGenome:SGD:S000005192; VEuPathDB:YNL248C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to RNA polymerase I activity. Involved in nucleolar large rRNA transcription by RNA polymerase I and transcription elongation from RNA polymerase I promoter. Located in nucleolus. Part of RNA polymerase I complex. Orthologous to human POLR1E (RNA polymerase I subunit E). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RPA49 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (181361..182608, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that the RNA polymerase I (Pol I) I transcription initiation cycle allows comparisons with promoter opening by Pol II and Pol III.
Title: Molecular insight into RNA polymerase I promoter recognition and promoter melting.
These results indicate that Rpa49, Rrn3 and Mot1 directly or indirectly influence biogenesis
Title: Identification of factors that promote biogenesis of tRNA(CGA)(Ser).
Core Factor's intrinsic mobility correlates well with different conformational states of the Pol I cleft, in addition to the stabilization of either Rrn7 N-terminal domain near Pol I wall or the tandem winged helix domain of A49 at a partially overlapping location.
Title: Structural mechanism of ATP-independent transcription initiation by RNA polymerase I.
yeast A34.5/A49 and mammalian PAF49/PAF53 heterodimeric complexes interact through species-specific sequence elements that appear to assume similar structural motifs.
Title: Characterization of the interactions of mammalian RNA polymerase I associated proteins PAF53 and PAF49.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to RNA polymerase I activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleotidyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in RNA polymerase I preinitiation complex assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleolar large rRNA transcription by RNA polymerase I	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nucleolar large rRNA transcription by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell size	HMP more info	PubMed
involved_in ribosome biogenesis	IEA Inferred from Electronic Annotation more info
involved_in transcription by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription elongation by RNA polymerase I	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription elongation by RNA polymerase I	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase I complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase I complex	IDA Inferred from Direct Assay more info	PubMed
part_of RNA polymerase I complex	IMP Inferred from Mutant Phenotype more info	PubMed
located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-directed RNA polymerase I subunit RPA49

NP_014151.1

RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

181361..182608 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183086.1 → NP_014151.1 TPA: DNA-directed RNA polymerase I subunit RPA49 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014151.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0U5, Q01080

UniProtKB/TrEMBL

A0A7I9G2N8, A6ZRJ5, B3LP88, B5VQG1, C7GPB3, C8ZFY9, G2WLL8, N1P524

Conserved Domains (1) summary

pfam06870
Location:33 → 411

RNA_pol_I_A49; A49-like RNA polymerase I associated factor

Nucleotide		Protein
Heading	Accession and Version	Protein

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
5LMX_M.1
5M3F_M.1
5M3M_M.1
5M5W_M.1
5N60_M.1
5N61_M.1
5OA1_M.1
5W5Y_M.1
5W64_M.1
5W65_M.1
5W66_M.1
6H67_M.1
6H68_M.1
6HKO_M.1
Q01080.2