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    SPT8 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850744, updated on 22-Aug-2024

    Summary

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    Official Symbol
    SPT8
    Primary source
    FungiDB:YLR055C
    Locus tag
    YLR055C
    See related
    SGD:S000004045; AllianceGenome:SGD:S000004045; VEuPathDB:YLR055C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables TBP-class protein binding activity and transcription coregulator activity. Involved in chromatin organization; histone acetylation; and regulation of transcription by RNA polymerase II. Located in nucleus. Part of SAGA complex. [provided by Alliance of Genome Resources, Apr 2022]
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    Genomic context

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    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (251272..253080, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Nrs1p Neighboring gene Osw2p Neighboring gene C-5 sterol desaturase Neighboring gene putative mannosidase MNL2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Characterization of new Spt3 and TATA-binding protein mutants of Saccharomyces cerevisiae: Spt3 TBP allele-specific interactions and bypass of Spt8. Laprade L, et al. Genetics, 2007 Dec. PMID 18073420, Free PMC Article
    2. The Saccharomyces cerevisiae SPT8 gene encodes a very acidic protein that is functionally related to SPT3 and TATA-binding protein. Eisenmann DM, et al. Genetics, 1994 Jul. PMID 8088510, Free PMC Article
    3. Three genes are required for trans-activation of Ty transcription in yeast. Winston F, et al. Genetics, 1987 Apr. PMID 3034719, Free PMC Article
    4. Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription. Shao W, et al. Nucleic Acids Res, 2020 Jun 19. PMID 32399566, Free PMC Article
    5. Suppression of Mediator is regulated by Cdk8-dependent Grr1 turnover of the Med3 coactivator. Gonzalez D, et al. Proc Natl Acad Sci U S A, 2014 Feb 18. PMID 24550274, Free PMC Article

    See all (165) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription.
      Title: Prp5-Spt8/Spt3 interaction mediates a reciprocal coupling between splicing and transcription.
    2. SAGA-TBP binding involves a network of interactions between subunits Spt3, Spt8, Spt20, and Spt7.
      Title: Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex.
    3. new spt15 mutations partially bypass an spt8 null mutation.
      Title: Characterization of new Spt3 and TATA-binding protein mutants of Saccharomyces cerevisiae: Spt3 TBP allele-specific interactions and bypass of Spt8.
    4. Data show that two components of the RNA polymerase II machinery, Med13 (Srb9) and Spt8, are two-hybrid partners of the conserved TFIIS N-terminal domain.
      Title: Members of the SAGA and Mediator complexes are partners of the transcription elongation factor TFIIS.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables TBP-class protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables TBP-class protein binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables transcription coregulator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in chromatin organization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of transcription by RNA polymerase II IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in negative regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of transcription by RNA polymerase II IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in ribosomal large subunit biogenesis IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Component Evidence Code Pubs
    part_of PeBoW complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of SAGA complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus HDA PubMed 
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of preribosome, large subunit precursor IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  

    General protein information

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    Preferred Names
    SAGA complex subunit SPT8
    NP_013156.1
    • Subunit of the SAGA transcriptional regulatory complex; not present in SAGA-like complex SLIK/SALSA; required for SAGA-mediated inhibition at some promoters

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      251272..253080 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001181942.1 → NP_013156.1  TPA: SAGA complex subunit SPT8 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013156.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VY57, P38915
      UniProtKB/TrEMBL
      G2WIP8
      Conserved Domains (2) summary
      sd00039
      Location:180 → 218
      7WD40; WD40 repeat [structural motif]
      cl29593
      Location:304 → 593
      WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P38915.1

    Gene LinkOut

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