EAF3 Eaf3p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: EAF3
Gene description: Eaf3p
Primary source: FungiDB:YPR023C
Locus tag: YPR023C
See related: SGD:S000006227; AllianceGenome:SGD:S000006227; VEuPathDB:YPR023C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables methylated histone binding activity. Involved in histone deacetylation; nucleic acid metabolic process; and regulation of nucleobase-containing compound metabolic process. Part of NuA4 histone acetyltransferase complex and Rpd3S/Clr6-CII complex. Human ortholog(s) of this gene implicated in Basilicata-Akhtar syndrome. Orthologous to several human genes including MORF4L1 (mortality factor 4 like 1) and MORF4L2 (mortality factor 4 like 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See EAF3 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (608826..610031, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Eaf3 chromodomain acts as a pH sensor for gene expression by altering its binding affinity for histone methylated-lysine residues.
Title: The Eaf3 chromodomain acts as a pH sensor for gene expression by altering its binding affinity for histone methylated-lysine residues.
Eaf3 is recruited to intron-containing genes and that Eaf3 interacts with the splicing factor Prp45. Eaf3 acts with Prp45 and Prp19 after formation of the precatalytic B complex around the time of splicing activation, thus revealing the step in splicing that is regulated by H3K36 methylation.
Title: H3K36 Methylation and the Chromodomain Protein Eaf3 Are Required for Proper Cotranscriptional Spliceosome Assembly.
Results demonstrate that the combination of Eaf3 and Yng2 interactions with their respective methyl marks on histone H3 is not important for NuA4 localization on the genome but is required for proper H4 acetylation throughout the transcription unit.
Title: Combined Action of Histone Reader Modules Regulates NuA4 Local Acetyltransferase Function but Not Its Recruitment on the Genome.
In vitro binding assays showed that Eaf3/5/7 specifically stimulates NuA4 binding to di- and trimethylated histone H3 Lys-36 and that this binding is important for NuA4 occupancy in transcribed ORFs.
Title: The Eaf3/5/7 Subcomplex Stimulates NuA4 Interaction with Methylated Histone H3 Lys-36 and RNA Polymerase II.
analysis of the molecular mechanism of the recognition and binding of the methylated H3K36 by Eaf3 provides new insights into the functional roles of the Eaf3 chromo domain
Title: Molecular basis of the interaction of Saccharomyces cerevisiae Eaf3 chromo domain with methylated H3K36.
The association of Rpd3S with chromatin requires its Eaf3 subunit, which binds histone H3 methylated at lysine 36.
Title: Structural basis for the recognition of methylated histone H3K36 by the Eaf3 subunit of histone deacetylase complex Rpd3S.
Data show that Eaf3 interaction with methylated histone H3 links histone deacetylation to RNA polymerase II elongation.
Title: Eaf3 chromodomain interaction with methylated H3-K36 links histone deacetylation to Pol II elongation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables methylated histone binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of antisense RNA transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of RNA stability	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription elongation by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of NuA4 histone acetyltransferase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of NuA4 histone acetyltransferase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Rpd3S complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Rpd3S complex	IDA Inferred from Direct Assay more info	PubMed
part_of histone acetyltransferase complex	IDA Inferred from Direct Assay more info	PubMed
part_of histone acetyltransferase complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Eaf3p

NP_015348.1

Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

608826..610031 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184120.1 → NP_015348.1 TPA: Eaf3p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015348.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W433, Q12432

UniProtKB/TrEMBL

A0A8H4F719, A6ZWR6, B3LL84, B5VTI0, C7GQB5, C8ZJ48, N1NW06

Conserved Domains (2) summary

pfam05712
Location:198 → 378

MRG

cl28914
Location:13 → 105

CD_CSD; CHROMO (CHRromatin Organization Modifier) domains and chromo shadow domains