RNF128 ring finger protein 128 [Homo sapiens (human)] - Gene

Summary

Official Symbol: RNF128provided by HGNC
Official Full Name: ring finger protein 128provided by HGNC
Primary source: HGNC:HGNC:21153
See related: Ensembl:ENSG00000133135 MIM:300439; AllianceGenome:HGNC:21153
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GRAIL
Summary: The protein encoded by this gene is a type I transmembrane protein that localizes to the endocytic pathway. This protein contains a RING zinc-finger motif and has been shown to possess E3 ubiquitin ligase activity. Expression of this gene in retrovirally transduced T cell hybridoma significantly inhibits activation-induced IL2 and IL4 cytokine production. Induced expression of this gene was observed in anergic CD4(+) T cells, which suggested a role in the induction of anergic phenotype. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Expression: Broad expression in small intestine (RPKM 27.8), liver (RPKM 27.8) and 15 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: Xq22.3

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	X	NC_000023.11 (106693838..106797016)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	X	NC_060947.1 (105130281..105233534)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	X	NC_000023.10 (105937068..106040246)

Chromosome X - NC_000023.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Mechanism of tumor-derived extracellular vesicles in prostatic cancer progression through the circFMN2/KLF2/RNF128 axis.
Title: Mechanism of tumor-derived extracellular vesicles in prostatic cancer progression through the circFMN2/KLF2/RNF128 axis.
RNF128 expression in lung adenocarcinoma is a favorable prognostic factor associated with decreased tumor-associated macrophages.
Title: RNF128 expression in lung adenocarcinoma is a favorable prognostic factor associated with decreased tumor-associated macrophages.
RING finger protein 128 (RNF128) regulates malignant biological behaviors of colorectal cancer cells via PI3K/AKT signaling pathway.
Title: RING finger protein 128 (RNF128) regulates malignant biological behaviors of colorectal cancer cells via PI3K/AKT signaling pathway.
UBCH5 Family Members Differentially Impact Stabilization of Mutant p53 via RNF128 Iso1 During Barrett's Progression to Esophageal Adenocarcinoma.
Title: UBCH5 Family Members Differentially Impact Stabilization of Mutant p53 via RNF128 Iso1 During Barrett's Progression to Esophageal Adenocarcinoma.
GRAIL inhibits the growth, migration and invasion of lung adenocarcinoma cells by modulating STAT3/C-MYC signaling pathways.
Title: GRAIL inhibits the growth, migration and invasion of lung adenocarcinoma cells by modulating STAT3/C-MYC signaling pathways.
E3 ubiquitin ligase Grail promotes hepatic steatosis through Sirt1 inhibition.
Title: E3 ubiquitin ligase Grail promotes hepatic steatosis through Sirt1 inhibition.
RNF128 downregulation was shown to correlate with the malignant phenotype of melanoma, and further functional assays demonstrated that low levels of RNF128 promoted melanoma progression via inducing cell epithelial-mesenchymal transition (EMT) and the acquisition of stemness.
Title: Downregulation of RNF128 activates Wnt/β-catenin signaling to induce cellular EMT and stemness via CD44 and CTTN ubiquitination in melanoma.
We found that isoform 2 of RNF128 is decreased in BE cells, resulting in increased levels of mutant p53, whereas isoform 1 of RNF128 is increased in BE cells, further promoting the stabilization of mutant p53.
Title: Isoforms of RNF128 Regulate the Stability of Mutant P53 in Barrett's Esophageal Cells.
Results demonstrate that Grail acts as a negative regulator of influenza A virus infection and replication by degrading viral nucleoprotein.
Title: Grail attenuates influenza A virus infection and pathogenesis by inhibiting viral nucleoprotein.
results show that expression of GRAIL is uniquely regulated by the specific miRNA in intestinal mucosa, and suggest that GRAIL may associate with the pathophysiology of Crohn's disease
Title: Regulation of anergy-related ubiquitin E3 ligase, GRAIL, in murine models of colitis and patients with Crohn's disease.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

DXS8048 (e-PCR), detects polymorphism
- UniSTS:40508

RH103289 (e-PCR)
- UniSTS:97620

SHGC-105584 (e-PCR)
- UniSTS:169335

RNF128_2495 (e-PCR)
- UniSTS:280966

SHGC-33056 (e-PCR)
- UniSTS:11920

DXS8097 (e-PCR)
- UniSTS:63693

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of cytokine production	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein catabolic process in the vacuole	IC Inferred by Curator more info	PubMed
involved_in protein localization to lysosome	IC Inferred by Curator more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein stability	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ubiquitin-dependent protein catabolic process	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cytosol	TAS Traceable Author Statement more info
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
is_active_in late endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase RNF128

Names: RING-type E3 ubiquitin transferase RNF128; gene related to anergy in lymphocytes protein; ring finger protein 128, E3 ubiquitin protein ligase

NP_078815.3

EC 2.3.2.27

NP_919445.1

EC 2.3.2.27

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_012576.1 RefSeqGene

Range

37864..108179

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_024539.3 → NP_078815.3 E3 ubiquitin-protein ligase RNF128 isoform 2

See identical proteins and their annotated locations for NP_078815.3

Status: REVIEWED

Description

Transcript Variant: This variant (2) has an alternate 5' end exon compared to variant 1. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1.

Source sequence(s)

AK027169, BC012931, BC036901

Consensus CDS

CCDS14520.1

UniProtKB/Swiss-Prot

Q8TEB7

Related

ENSP00000316127.3, ENST00000324342.7

Conserved Domains (2) summary

cd02122
Location:34 → 167

PA_GRAIL_like; PA _GRAIL_like: Protease-associated (PA) domain GRAIL-like. This group includes PA domain containing E3 (ubiquitin ligases) similar to human GRAIL (gene related to anergy in lymphocytes) protein. Proteins in this group contain a C3H2C3 RING finger. E3 ...

pfam13639
Location:249 → 292

zf-RING_2; Ring finger domain
NM_194463.2 → NP_919445.1 E3 ubiquitin-protein ligase RNF128 isoform 1 precursor

See identical proteins and their annotated locations for NP_919445.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

AK074264, BC012931, BI600046

Consensus CDS

CCDS14521.1

UniProtKB/Swiss-Prot

A0PJI4, Q6PH80, Q6ZTJ8, Q8TEB7, Q96RF3, Q9H5E4

Related

ENSP00000255499.2, ENST00000255499.3

Conserved Domains (2) summary

cd02122
Location:48 → 193

PA_GRAIL_like; PA _GRAIL_like: Protease-associated (PA) domain GRAIL-like. This group includes PA domain containing E3 (ubiquitin ligases) similar to human GRAIL (gene related to anergy in lymphocytes) protein. Proteins in this group contain a C3H2C3 RING finger. E3 ...

cd16802
Location:275 → 323

RING-H2_RNF128_like; RING finger, H2 subclass, found in RING finger protein 128 (RNF128) and similar proteins