RPN1 proteasome regulatory particle base subunit RPN1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPN1
Official Full Name: proteasome regulatory particle base subunit RPN1
Primary source: SGD:S000001069
Locus tag: YHR027C
See related: AllianceGenome:SGD:S000001069; FungiDB:YHR027C; VEuPathDB:YHR027C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: HRD2; NAS1
Summary: Enables protein-macromolecule adaptor activity. Predicted to be involved in proteasome-mediated ubiquitin-dependent protein catabolic process. Located in nucleus and proteasome storage granule. Part of proteasome regulatory particle, base subcomplex. Orthologous to human PSMD2 (proteasome 26S subunit ubiquitin receptor, non-ATPase 2). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See RPN1 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (161730..164711, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

A novel recognition site for polyubiquitin and ubiquitin-like signals in an unexpected region of proteasomal subunit Rpn1.
Title: A novel recognition site for polyubiquitin and ubiquitin-like signals in an unexpected region of proteasomal subunit Rpn1.
Polyubiquitin and ubiquitin-like signals share common recognition sites on proteasomal subunit Rpn1.
Title: Polyubiquitin and ubiquitin-like signals share common recognition sites on proteasomal subunit Rpn1.
The structures of proteasome substrate receptor complexes with the shuttle factors that deliver ubiquitinated proteins to proteasomes have been solved, namely human Rpn13 complexed with PLIC2 and Saccharomyces cerevisiae Rpn1 with Rad23.
Title: Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome.
Data suggest regulated process for removal of proteasomes upon nitrogen starvation; autophagy involves separate processes for dissociation and nuclear export of core (PRE2) and regulatory (RPN1) proteins; only core protein autophagy depends on UBP3.
Title: Starvation Induces Proteasome Autophagy with Different Pathways for Core and Regulatory Particles.
Study found that the five known proteasomal ubiquitin receptors in yeast are collectively nonessential for ubiquitin recognition and identified a sixth receptor, Rpn1. A site (T1:) in the Rpn1 toroid recognized ubiquitin and ubiquitin-like (UBL:) domains of substrate shuttling factors.
Title: Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome.
Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.
Title: Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.
The current study identifies residues in the LRR1 domain of Rpn1 that contribute to shuttle receptor docking. It validates Ddi1 as a proteasomal shuttle receptor whose stable binding to the proteasome depends on Rpn1 residue D517.
Title: Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1.
Comparison of the full-length Rpn1 and Rpn2 with the truncated proteins showed molecules similar in size and shape, indicating that their terminal regions are flexible and thus smeared to invisibility in the averaged images.
Title: Electron microscopic evidence in support of alpha-solenoid models of proteasomal subunits Rpn1 and Rpn2.
Rpn2 interfaces with the 20S, whereas Rpn1 sits atop Rpn2, serving as a docking site for a substrate-recruitment factor. The 19S ATPases encircle the Rpn1-Rpn2 stack, covering the remainder of the 20S surface.
Title: The central unit within the 19S regulatory particle of the proteasome.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to endopeptidase activity	TAS Traceable Author Statement more info	PubMed
enables enzyme regulator activity	IEA Inferred from Electronic Annotation more info
enables protein-macromolecule adaptor activity	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of protein catabolic process	IEA Inferred from Electronic Annotation more info
involved_in ubiquitin-dependent protein catabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome complex	IEA Inferred from Electronic Annotation more info
part_of proteasome regulatory particle, base subcomplex	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome regulatory particle, base subcomplex	IDA Inferred from Direct Assay more info	PubMed
is_active_in proteasome storage granule	IBA Inferred from Biological aspect of Ancestor more info
located_in proteasome storage granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: proteasome regulatory particle base subunit RPN1

NP_011892.1

Non-ATPase base subunit of the 19S RP of the 26S proteasome; may participate in the recognition of several ligands of the proteasome; contains a leucine-rich repeat (LRR) domain, a site for protein-protein interactions; RP is the acronym for regulatory particle

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

161730..164711 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179157.1 → NP_011892.1 TPA: proteasome regulatory particle base subunit RPN1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011892.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DKX4, P38764

UniProtKB/TrEMBL

C7GTV3, N1P553

Conserved Domains (1) summary

COG5110
Location:1 → 993

RPN1; 26S proteasome regulatory complex component [Posttranslational modification, protein turnover, chaperones]