RPN4 stress-regulated transcription factor RPN4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: RPN4
Gene description: stress-regulated transcription factor RPN4
Primary source: FungiDB:YDL020C
Locus tag: YDL020C
See related: SGD:S000002178; AllianceGenome:SGD:S000002178; VEuPathDB:YDL020C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SON1; UFD5
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in negative regulation of transcription by RNA polymerase II; positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance; and regulation of DNA repair. Located in nucleus. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RPN4 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (415113..416708, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Impairment of RPN4, a transcription factor, induces ER stress and lipid abnormality in Saccharomyces cerevisiae.
Title: Impairment of RPN4, a transcription factor, induces ER stress and lipid abnormality in Saccharomyces cerevisiae.
[Rpn4p without the DNA-Binding Domain Provides Saccharomyces cerevisiae Resistance to Oxidative Stress and Cycloheximide].
Title: [Rpn4p without the DNA-Binding Domain Provides Saccharomyces cerevisiae Resistance to Oxidative Stress and Cycloheximide].
The authors identify the proteasome biogenesis regulator Rpn4 and show that it cooperates with the unfolded protein response. Rpn4 abundance increases during endoplasmic reticulum stress, first by a post-transcriptional, then by a transcriptional mechanism.
Title: The proteasome biogenesis regulator Rpn4 cooperates with the unfolded protein response to promote ER stress resistance.
Rpn4 induces TMC1 transcription in response to misfolded proteins. However, this response is counteracted by rapid proteasome-dependent degradation of Tmc1, which serves to normalize Tmc1 protein levels after induction
Title: Tmc1 Is a Dynamically Regulated Effector of the Rpn4 Proteotoxic Stress Response.
Rpn4-responsive elements in genes related to proteasome functions
Title: Identification of minimum Rpn4-responsive elements in genes related to proteasome functions.
under the methyl methanesulfonate stress Rpn4 promotes the regulation of several genes involved in DNA repair, antioxidant response and glucose metabolism.
Title: [RPN4 the yeast transcription factor promotes the complex defence against methyi, methanesulfonate].
the ubiquitin-independent degradation of Rpn4 requires the 19S regulatory particle.
Title: The N-terminal domain of Rpn4 serves as a portable ubiquitin-independent degron and is recognized by specific 19S RP subunits.
evidence for the role of proteasomal degradation of Rpn4 in nonhomologous end-joining
Title: Inhibition of proteasomal degradation of rpn4 impairs nonhomologous end-joining repair of DNA double-strand breaks.
Intracellular proteasome activity is correlated with the transactivation potency of Rpn4.
Title: The transcription activation domain of Rpn4 is separate from its degrons.
Rpn4 thus represents an important stress-responsive mediator whose degradation as well as availability are critical for cell survival under stressed conditions.
Title: Proteasomal degradation of Rpn4 in Saccharomyces cerevisiae is critical for cell viability under stressed conditions.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to arsenic-containing substance	HMP more info	PubMed
involved_in cellular response to arsenic-containing substance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to stress	HMP more info	PubMed
involved_in cellular response to stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	HMP more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	HMP more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: stress-regulated transcription factor RPN4

NP_010264.3

Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

415113..416708 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180079.3 → NP_010264.3 TPA: stress-regulated transcription factor RPN4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010264.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRX0, E9P944, Q03465

UniProtKB/TrEMBL

A0A8H8UMQ4, C7GW59, C8Z4P9, N1P5X6

Conserved Domains (1) summary

sd00017
Location:450 → 468

ZF_C2H2; C2H2 Zn finger [structural motif]