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    PCA1 cation-transporting P-type ATPase PCA1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 852598, updated on 28-Oct-2024

    Summary

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    Official Symbol
    PCA1
    Official Full Name
    cation-transporting P-type ATPase PCA1
    Primary source
    SGD:S000000499
    Locus tag
    YBR295W
    See related
    AllianceGenome:SGD:S000000499; FungiDB:YBR295W; VEuPathDB:YBR295W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    CAD2; PAY2
    Summary
    Enables P-type cadmium transporter activity; copper ion binding activity; and proteasome binding activity. Involved in cadmium ion transport; intracellular monoatomic cation homeostasis; and transmembrane transport. Located in plasma membrane. Part of proteasome core complex. [provided by Alliance of Genome Resources, Oct 2024]
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    Genomic context

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    See PCA1 in Genome Data Viewer
    Location:
    chromosome: II
    Exon count:
    1
    Sequence:
    Chromosome: II; NC_001134.8 (792849..796499)

    Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene Vba2p Neighboring gene sulfate permease Neighboring gene Pho89p Neighboring gene Tyc1p

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001134.8

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Resistance to cadmium is under control of the CAD2 gene in the yeast Saccharomyces cerevisiae. Tohoyama H, et al. Curr Genet, 1990 Oct. PMID 2249249
    2. Variation of the response to metal ions and nonsense-mediated mRNA decay across different Saccharomyces cerevisiae genetic backgrounds. Wong A, et al. Yeast, 2021 Sep. PMID 33955055, Free PMC Article
    3. A putative P-type Cu(2+)-transporting ATPase gene on chromosome II of Saccharomyces cerevisiae. Rad MR, et al. Yeast, 1994 Sep. PMID 7754711
    4. The cadmium-resistant gene, CAD2, which is a mutated putative copper-transporter gene (PCA1), controls the intracellular cadmium-level in the yeast S. cerevisiae. Shiraishi E, et al. Curr Genet, 2000 Feb. PMID 10743563
    5. A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae. Adle DJ, et al. J Biol Chem, 2007 Jan 12. PMID 17107946, Free PMC Article

    See all (34) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Determined the roles for the molecular factors of ERAD in dislodging Pca1p from the endoplasmic reticulum (ER). Inactivation of the 20S proteasome leads to accumulation of ubiquitinated Pca1p in the ER membrane, suggesting a role for the proteasome in extraction of Pca1p from the ER.
      Title: Endoplasmic Reticulum-associated Degradation of Pca1p, a Polytopic Protein, via Interaction with the Proteasome at the Membrane.
    2. Data indicate that specific cysteine residues in a degron of cation-transporting P-type ATPase PCA1 (Pca1p) sense cadmium.
      Title: Cadmium and Secondary Structure-dependent Function of a Degron in the Pca1p Cadmium Exporter.
    3. cadmium resistance is primarily caused by an enhanced function of a metal efflux pump, PCA1.
      Title: A tradeoff drives the evolution of reduced metal resistance in natural populations of yeast.
    4. Direct cadmium sensing at the endoplasmic reticulum by a degron in Pca1 leads to an escape of Pca1 from endoplasmic reticulum-associated degradation.
      Title: Cadmium-mediated rescue from ER-associated degradation induces expression of its exporter.
    5. Cadmium sensing by cysteine residues within this domain circumvents ubiquitination and degradation of Pca1
      Title: Expressional control of a cadmium-transporting P1B-type ATPase by a metal sensing degradation signal.
    6. Overexpression or deletion of the functional allele in yeast demonstrates that PCA1 is a cadmium efflux pump
      Title: A cadmium-transporting P1B-type ATPase in yeast Saccharomyces cerevisiae.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATP hydrolysis activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ATPase-coupled monoatomic cation transmembrane transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables P-type cadmium transporter activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables P-type cadmium transporter activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables P-type cadmium transporter activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables copper ion binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables copper ion binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables proteasome binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cadmium ion transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in cadmium ion transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in detoxification of copper ion IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in intracellular monoatomic cation homeostasis IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in monoatomic cation transmembrane transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in monoatomic cation transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in monoatomic ion transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to cadmium ion IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transmembrane transport IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in transmembrane transport IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in plasma membrane IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in plasma membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of proteasome core complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    cation-transporting P-type ATPase PCA1
    NP_009854.2
    • Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001134.8 Reference assembly

      Range
      792849..796499
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001178643.2 → NP_009854.2  TPA: cation-transporting P-type ATPase PCA1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_009854.2

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VQT9, P38360
      UniProtKB/TrEMBL
      A6ZLN2, B3LML9, C7GMT8, N1PAW9
      Conserved Domains (2) summary
      cd00371
      Location:413 → 471
      HMA; Heavy-metal-associated domain (HMA) is a conserved domain of approximately 30 amino acid residues found in a number of proteins that transport or detoxify heavy metals, for example, the CPx-type heavy metal ATPases and copper chaperones. HMA domain ...
      TIGR01511
      Location:605 → 1193
      ATPase-IB1_Cu; copper-(or silver)-translocating P-type ATPase
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P38360.2

    Gene LinkOut

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