PDR3 drug-responsive transcription factor PDR3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PDR3
Official Full Name: drug-responsive transcription factor PDR3
Primary source: SGD:S000000101
Locus tag: YBL005W
See related: AllianceGenome:SGD:S000000101; FungiDB:YBL005W; VEuPathDB:YBL005W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AMY2; TPE2
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in cellular response to xenobiotic stimulus; positive regulation of cellular response to drug; and positive regulation of transcription by RNA polymerase II. Located in nucleus. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See PDR3 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (217470..220400)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our results demonstrated that organic solvent stress was likely to damage mitochondria, causing generation of reactive oxygen species and mitochondrial fragmentation, and to activate retrograde signaling pathway via Pdr3p to upregulate PDR5 expression.
Title: Activation of the mitochondrial signaling pathway in response to organic solvent stress in yeast.
site-directed mutagenesis of Asp853 in Pdr3p; results indicate that, while Pdr3p can tolerate several substitutions of Asp853, the occurrence of a hydrophobic amino acid at this position has an adverse effect on its function
Title: Site-directed mutagenesis of Asp853 in Pdr3p transcriptional activator from Saccharomyces cerevisiae.
Overproduction of Psd1 in wild-type ([rho(+)]) cells was found to induce PDR5 transcription and drug resistance in a Pdr3-dependent manner.
Title: Evidence for the bifunctional nature of mitochondrial phosphatidylserine decarboxylase: role in Pdr3-dependent retrograde regulation of PDR5 expression.
compounds with potential membrane-damaging or -perturbing effects might function as an activating signal for Pdr1 and Pdr3
Title: Membrane-active compounds activate the transcription factors Pdr1 and Pdr3 connecting pleiotropic drug resistance and membrane lipid homeostasis in saccharomyces cerevisiae.
This activation of FLR1 transcription is fully dependent on Yap1p and is reduced (by 50%) in the absence of Rpn4p, Yrr1p or Pdr3p.
Title: Yeast adaptation to mancozeb involves the up-regulation of FLR1 under the coordinate control of Yap1, Rpn4, Pdr3, and Yrr1.
The transcriptional activation of TPO1 and PDR5 was registered after exposure of the unadapted yeast population to acute artesunate-induced stress, being significantly reduced in the absence of Pdr1p and abolished in the absence of Pdr1p and Pdr3p.
Title: Adaptive response to the antimalarial drug artesunate in yeast involves Pdr1p/Pdr3p-mediated transcriptional activation of the resistance determinants TPO1 and PDR5.
These results demonstrate that amino acid residue Asp853 is essential for Pdr3p function, and indicate that specific loss-of-function pdr3 mutations can convert the Pdr3p transcription activator to a multicopy suppressor of multidrug resistance.
Title: Loss-of-function pdr3 mutations convert the Pdr3p transcription activator to a protein suppressing multidrug resistance in Saccharomyces cerevisiae.
Pdr3p activation of RSB1 transcription is an important feature of the retrograde response allowing normal detoxification of an endogenous sphingolipid precursor.
Title: Long chain base tolerance in Saccharomyces cerevisiae is induced by retrograde signals from the mitochondria.
We show that HSF activates expression of PDR3, encoding a multidrug resistance (MDR) transcription factor that also directly activates RPN4 gene expression.
Title: A stress regulatory network for co-ordinated activation of proteasome expression mediated by yeast heat shock transcription factor.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in cellular response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cellular response to drug	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cellular response to drug	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: drug-responsive transcription factor PDR3

NP_009548.1

Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); has a role in response to drugs and organic solvents; post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

217470..220400

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178245.1 → NP_009548.1 TPA: drug-responsive transcription factor PDR3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009548.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPZ5, E9P920, P33200

UniProtKB/TrEMBL

G2W904

Conserved Domains (2) summary

smart00066
Location:9 → 49

GAL4; GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain

pfam04082
Location:446 → 601

Fungal_trans; Fungal specific transcription factor domain