DUG3 glutamine amidotransferase subunit DUG3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: DUG3
Gene description: glutamine amidotransferase subunit DUG3
Primary source: FungiDB:YNL191W
Locus tag: YNL191W
See related: SGD:S000005135; AllianceGenome:SGD:S000005135; VEuPathDB:YNL191W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables omega peptidase activity. Contributes to glutathione hydrolase activity. Involved in glutathione catabolic process. Located in cytoplasm. Part of glutathione hydrolase complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See DUG3 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (280432..281505)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dug2p and Dug3p were required together for the cleavage of glutathione into glutamate and Cys-Gly.
Title: Glutathione degradation by the alternative pathway (DUG pathway) in Saccharomyces cerevisiae is initiated by (Dug2p-Dug3p)2 complex, a novel glutamine amidotransferase (GATase) enzyme acting on glutathione.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables amidophosphoribosyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed
contributes_to glutathione hydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables omega peptidase activity	IBA Inferred from Biological aspect of Ancestor more info
enables omega peptidase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables peptidase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in glutamine metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glutathione catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutathione catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glutathione catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of glutathione hydrolase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of glutathione hydrolase complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: glutamine amidotransferase subunit DUG3

NP_014208.1

Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

280432..281505

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183029.1 → NP_014208.1 TPA: glutamine amidotransferase subunit DUG3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014208.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0Z6, P53871

UniProtKB/TrEMBL

A0A6A5Q757, A6ZRP6, B3LP40, B5VQL9, C7GTG3, C8ZG39, G2WLR9, N1NWF5

Conserved Domains (1) summary

cd01908
Location:1 → 292

YafJ; Glutamine amidotransferases class-II (Gn-AT)_YafJ-type. YafJ is a glutamine amidotransferase-like protein of unknown function found in prokaryotes, eukaryotes and archaea. YafJ has a conserved structural fold similar to those of other class II ...