RHO1 Rho family GTPase RHO1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RHO1
Official Full Name: Rho family GTPase RHO1
Primary source: SGD:S000006369
Locus tag: YPR165W
See related: AllianceGenome:SGD:S000006369; FungiDB:YPR165W; VEuPathDB:YPR165W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables G-protein beta-subunit binding activity and GTPase activity. Involved in several processes, including cytoskeleton organization; positive regulation of protein kinase C signaling; and regulation of mitotic cytokinetic process. Located in several cellular components, including cellular bud tip; fungal-type vacuole membrane; and mating projection tip. Part of 1,3-beta-D-glucan synthase complex and incipient cellular bud site. Used to study ulcerative colitis. Human ortholog(s) of this gene implicated in breast carcinoma. Orthologous to several human genes including RHOA (ras homolog family member A). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RHO1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (875368..875997)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Authors propose that Rho1 and Pkc1 coordinate septin ring assembly with membrane and cell wall remodeling partly by controlling Syp1 residence at the bud neck.
Title: Rho1- and Pkc1-dependent phosphorylation of the F-BAR protein Syp1 contributes to septin ring assembly.
These results suggested that the tumour suppressor homologue SRO7/SRO77 regulated cell proliferation and yeast colony development via the Rho1-Tor1 pathway.
Title: Sro7 and Sro77, the yeast homologues of the Drosophila lethal giant larvae (Lgl), regulate cell proliferation via the Rho1-Tor1 pathway.
the Rho1p-eEF1A complex acts to spatially localize a pool of eEF1A to vacuoles where it can readily organize F-actin.
Title: Yeast translation elongation factor-1A binds vacuole-localized Rho1p to facilitate membrane integrity through F-actin remodeling.
The signaling network of Rho1 extends into many TORC1-dependent processes and sheds light on how yeast cells coordinate polarized spatial expansion with mass increase.
Title: TOR under stress: targeting TORC1 by Rho1 GTPase.
synthesis of the cell wall through partitioning glucose to the periphery and the formation of the signaling complex with Rom2 and Ssd1 to promote Rho1-dependent polarized cell growth.
Title: PAS kinase promotes cell survival and growth through activation of Rho1.
TOR complex 1 acts both upstream and downstream of Rho1 GTPase, unveiling a mechanism that integrates stress and nutrient signals to coordinate Rho1-mediated spatial expansion and TORC1-dependent mass increase.
Title: The TOR complex 1 is a direct target of Rho1 GTPase.
The result reveal the existence of a second pathway for endocytosis in yeast, which suggests similarities with the RhoA-dependent endocytic pathways of mammalian cells.
Title: Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin.
Rho1 protects yeast cells from oxidative stress by regulating multiple downstream targets including Ycf1.
Title: The Rho1 GTPase acts together with a vacuolar glutathione S-conjugate transporter to protect yeast cells from oxidative stress.
Mtl1 has an essential role in signaling oxidative stress and quiescence to the CWI pathway and to the general stress response through Rho1 and the inhibition of either the TOR1 or RAS2 functions
Title: Mtl1 is required to activate general stress response through Tor1 and Ras2 inhibition under conditions of glucose starvation and oxidative stress.
These results define unique activation mechanisms for Cdc42p and Rho1p, which may be linked to the vacuole membrane fusion mechanism.
Title: Cdc42p and Rho1p are sequentially activated and mechanistically linked to vacuole membrane fusion.

Submit: New GeneRIF Correction See all GeneRIFs (18)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables G-protein beta-subunit binding	IPI Inferred from Physical Interaction more info	PubMed
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables enzyme activator activity	IDA Inferred from Direct Assay more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in actin cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin cytoskeleton organization	IPI Inferred from Physical Interaction more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in budding cell bud growth	IGI Inferred from Genetic Interaction more info	PubMed
involved_in budding cell bud growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular bud neck septin ring organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mitotic actomyosin contractile ring assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of mitotic actomyosin contractile ring assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of cell size	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell wall (1->3)-beta-D-glucan biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell wall (1->3)-beta-D-glucan biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of exocyst localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of fungal-type cell wall organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of secondary cell septum biogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of vacuole fusion, non-autophagic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in small GTPase-mediated signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info
involved_in small GTPase-mediated signal transduction	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of 1,3-beta-D-glucan synthase complex	IDA Inferred from Direct Assay more info	PubMed
located_in Golgi apparatus	IDA Inferred from Direct Assay more info	PubMed
located_in cell periphery	HDA more info	PubMed
located_in cellular bud	HDA more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial outer membrane	HDA more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in peroxisomal membrane	IEA Inferred from Electronic Annotation more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	HDA more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Rho family GTPase RHO1

NP_015491.1

EC 3.6.5.2

GTP-binding protein of the rho subfamily of Ras-like small GTPases; involved in establishment of cell polarity; regulates protein kinase C (Pkc1p); positive regulatory subunit of the 1,3-beta-D-glucan synthase complex, activating 1,3-beta-glucan synthase (Fks1p and Gsc2p), an enzyme involved in cell wall biosynthesis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

875368..875997

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184262.1 → NP_015491.1 TPA: Rho family GTPase RHO1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015491.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W4G6, P06780

UniProtKB/TrEMBL

A6ZX41, B3LKC6, B5VTV0, C7GP48, C8ZJJ1, G2WPX8, N1P2G9

Conserved Domains (1) summary

cd01870
Location:10 → 184

RhoA_like; Ras homology family A (RhoA)-like includes RhoA, RhoB and RhoC