SPN1 Spn1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SPN1
Official Full Name: Spn1p
Primary source: SGD:S000006337
Locus tag: YPR133C
See related: AllianceGenome:SGD:S000006337; FungiDB:YPR133C; VEuPathDB:YPR133C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: IWS1
Summary: Enables RNA polymerase II complex binding activity; RNA polymerase II-specific DNA-binding transcription factor binding activity; and chromatin binding activity. Involved in nucleosome assembly; poly(A)+ mRNA export from nucleus; and regulation of DNA-templated transcription. Part of RNA polymerase II, core complex. Orthologous to human IWS1 (interacts with SUPT6H, CTD assembly factor 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SPN1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (795978..797210, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Suppressor mutations that make the essential transcription factor Spn1/Iws1 dispensable in Saccharomyces cerevisiae.
Title: Suppressor mutations that make the essential transcription factor Spn1/Iws1 dispensable in Saccharomyces cerevisiae.
Spn1 and Its Dynamic Interactions with Spt6, Histones and Nucleosomes.
Title: Spn1 and Its Dynamic Interactions with Spt6, Histones and Nucleosomes.
The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae.
Title: The conserved elongation factor Spn1 is required for normal transcription, histone modifications, and splicing in Saccharomyces cerevisiae.
Spn1 is a histone binding factor with histone chaperone functions.
Title: The elongation factor Spn1 is a multi-functional chromatin binding protein.
This provides evidence for a role of wild-type Spn1 in promoting genome instability, as well as having ties to overcoming replication stress and contributing to chronological aging.
Title: Genome Instability Is Promoted by the Chromatin-Binding Protein Spn1 in Saccharomyces cerevisiae.
Study reports the crystal structure of the conserved central domain of Saccharomyces cerevisiae Spn1; the central domain is composed of 8 alpha-helices in a right-handed superhelical arrangement and exhibits structural similarity to domain I of TFIIS.
Title: The transcription factor Spn1 regulates gene expression via a highly conserved novel structural motif.
These findings link Spn1 functions to the transition from an inactive to an actively transcribing RNAPII complex at a postrecruitment-regulated promoter.
Title: Spn1 regulates the recruitment of Spt6 and the Swi/Snf complex during transcriptional activation by RNA polymerase II.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA polymerase II complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II-specific DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in nucleosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in poly(A)+ mRNA export from nucleus	IBA Inferred from Biological aspect of Ancestor more info
involved_in poly(A)+ mRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulation of transcription elongation by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of RNA polymerase II, core complex	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IC Inferred by Curator more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Spn1p

NP_015458.1

Protein involved in RNA polymerase II transcription; also required for histone modifications and splicing; constitutively recruited to the CYC1 promoter and required for recruitment of chromatin remodeling factors for the expression of CYC1 gene; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain is highly conserved throughout eukaryotes; mutations confer an Spt- phenotype

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

795978..797210 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001184230.1 → NP_015458.1 TPA: Spn1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015458.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W4C9, Q06505

UniProtKB/TrEMBL

A6ZX10, B3LK94, B5VTR8, C8ZJF5, N1NW54

Conserved Domains (1) summary

COG5139
Location:1 → 405

COG5139; Uncharacterized conserved protein [Function unknown]