PBP1 Pbp1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PBP1
Official Full Name: Pbp1p
Primary source: SGD:S000003410
Locus tag: YGR178C
See related: AllianceGenome:SGD:S000003410; FungiDB:YGR178C; VEuPathDB:YGR178C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: MRS16
Summary: Enables mRNA binding activity. Involved in several processes, including cellular response to glucose starvation; negative regulation of TORC1 signaling; and stress granule assembly. Located in cytoplasmic stress granule. Used to study amyotrophic lateral sclerosis type 13. Human ortholog(s) of this gene implicated in late onset Parkinson's disease and spinocerebellar ataxia type 2. Orthologous to human ATXN2 (ataxin 2) and ATXN2L (ataxin 2 like). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See PBP1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (851047..853215, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pbp1 associates with Puf3 and promotes translation of its target mRNAs involved in mitochondrial biogenesis.
Title: Pbp1 associates with Puf3 and promotes translation of its target mRNAs involved in mitochondrial biogenesis.
The Pbp1 methionine residues are sensitive to hydrogen peroxide mediated oxidation in vitro and in living cells. Methionine oxidation melts Pbp1 liquid-like droplets in a manner reversed by methionine sulfoxide reductase enzymes.
Title: Redox State Controls Phase Separation of the Yeast Ataxin-2 Protein via Reversible Oxidation of Its Methionine-Rich Low-Complexity Domain.
Pbp1 binds to TORC1 specifically during respiratory growth, but utilizes an additional methionine-rich, low complexity (LC) region to inhibit TORC1. This LC region causes phase separation, forms reversible fibrils, and enables self-association into assemblies required for TORC1 inhibition.
Title: Yeast Ataxin-2 Forms an Intracellular Condensate Required for the Inhibition of TORC1 Signaling during Respiratory Growth.
Quantitative global proteomics of yeast PBP1 deletion mutants and their stress responses identifies glucose metabolism, mitochondrial, and stress granule changes.
Title: Quantitative Global Proteomics of Yeast PBP1 Deletion Mutants and Their Stress Responses Identifies Glucose Metabolism, Mitochondrial, and Stress Granule Changes.
Snf1 phosphorylates and activates Psk1. Once activated, Psk1 may phosphorylate Pbp1, which is known to stimulate stress granule formation and inhibit TORC1 through sequestration. Thus, Psk1 is a link between energy status and cell growth/proliferation.
Title: PAS kinase is activated by direct SNF1-dependent phosphorylation and mediates inhibition of TORC1 through the phosphorylation and activation of Pbp1.
Pbp1 is involved in the Ccr4- and Khd1-mediated regulation of cell growth through the association with Rpl12a and Rpl12b.
Title: Pbp1 is involved in Ccr4- and Khd1-mediated regulation of cell growth through association with ribosomal proteins Rpl12a and Rpl12b.
Pbp1 and Dhh1 over-expression inhibits cell growth, and for Dhh1, leads to the accumulation of stress granules
Title: Localization to, and effects of Pbp1, Pbp4, Lsm12, Dhh1, and Pab1 on stress granules in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular response to glucose starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of rDNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TORC1 signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translation	IPI Inferred from Physical Interaction more info	PubMed
involved_in regulation of mitophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stress granule assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in stress granule assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
is_active_in cytoplasmic stress granule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Pbp1p

NP_011694.3

Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; regulates TORC1 signaling and autophagy; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; regulates expression of genes involved in gluconeogenesis and mitochondrial function through multiple mechanisms

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

851047..853215 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181307.3 → NP_011694.3 TPA: Pbp1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011694.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUW2, P53297

UniProtKB/TrEMBL

G2WEQ8, N1P9X7

Conserved Domains (1) summary

COG5180
Location:3 → 722

PBP1; PAB1-binding protein PBP1, interacts with poly(A)-binding protein [RNA processing and modification]