DOT1 histone methyltransferase DOT1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DOT1
Official Full Name: histone methyltransferase DOT1
Primary source: SGD:S000002848
Locus tag: YDR440W
See related: AllianceGenome:SGD:S000002848; FungiDB:YDR440W; VEuPathDB:YDR440W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: KMT4; PCH1
Summary: Enables histone methyltransferase activity (H3-K79 specific) and nucleosomal histone binding activity. Involved in several processes, including DNA repair; chromatin organization; and negative regulation of cell cycle phase transition. Located in nucleus. Human ortholog(s) of this gene implicated in mixed phenotype acute leukemia. Orthologous to human DOT1L (DOT1 like histone lysine methyltransferase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See DOT1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1342493..1344241)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regulation of the Dot1 histone H3K79 methyltransferase by histone H4K16 acetylation.
Title: Regulation of the Dot1 histone H3K79 methyltransferase by histone H4K16 acetylation.
Data show that histone methyltransferase DOT1 (Dot1)possesses chromatin regulatory functions that are independent of its methyltransferase activity.
Title: Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism.
and Dot1 cooperate to drive senescence
Title: TERRA and the histone methyltransferase Dot1 cooperate to regulate senescence in budding yeast.
Findings suggest that Dot1p-mediated histone chaperone activity controls nucleosome dynamics in transcribed regions.
Title: Dot1 regulates nucleosome dynamics by its inherent histone chaperone activity in yeast.
Dot1 histone methyltransferases share a distributive mechanism but have highly diverged catalytic properties
Title: Dot1 histone methyltransferases share a distributive mechanism but have highly diverged catalytic properties.
Dot1 has a role in histone H3K79 methylation that promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation
Title: Dot1-dependent histone H3K79 methylation promotes the formation of meiotic double-strand breaks in the absence of histone H3K4 methylation in budding yeast.
Dot1-dependent H3K79me also promotes Hop1 activation and its proper distribution along zip1 meiotic chromosomes, at least in part, by regulating Pch2 localization
Title: Dot1-dependent histone H3K79 methylation promotes activation of the Mek1 meiotic checkpoint effector kinase by regulating the Hop1 adaptor.
histone methyltransferase Dot1 mediates global genomic repair by methylating histone H3 on lysine 79.
Title: Evidence that the histone methyltransferase Dot1 mediates global genomic repair by methylating histone H3 on lysine 79.
loss of Dot1 did not globally alter Sir2 or Sir3 occupancy in subtelomeric regions, but only led to some telomere-specific changes
Title: Dot1 and histone H3K79 methylation in natural telomeric and HM silencing.
contributes to the regulation of DNA damage tolerance and modulates the response to the alkylating agent MMS through its catalytic activity on H3K79.
Title: Regulation of tolerance to DNA alkylating damage by Dot1 and Rad53 in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction See all GeneRIFs (20)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables histone H3K79 methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables histone H3K79 methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H3K79 methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone H3K79 methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables histone H3K79 trimethyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone binding	IEA Inferred from Electronic Annotation more info
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA damage checkpoint signaling	IEA Inferred from Electronic Annotation more info
involved_in DNA damage checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in global genome nucleotide-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic recombination checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in mitotic G1 DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleosome assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in nucleotide-excision repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in nucleotide-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in postreplication repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in recombinational repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in recombinational repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in subtelomeric heterochromatin formation	IEA Inferred from Electronic Annotation more info
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
part_of nucleosome	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: histone methyltransferase DOT1

NP_010728.1

EC 2.1.1.360

Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response; single DOT1 gene produces two Dot1p isoforms from alternative translation start sites as a result of leaky scanning by the ribosome

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1342493..1344241

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180748.1 → NP_010728.1 TPA: histone methyltransferase DOT1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010728.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VT67, Q04089

UniProtKB/TrEMBL

B3LFL9, C7GTP3, N1P5M2

Conserved Domains (1) summary

pfam08123
Location:350 → 555

DOT1; Histone methylation protein DOT1