SUP45 translation termination factor eRF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SUP45
Official Full Name: translation termination factor eRF1
Primary source: SGD:S000000347
Locus tag: YBR143C
See related: AllianceGenome:SGD:S000000347; FungiDB:YBR143C; VEuPathDB:YBR143C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SAL4; SUP1; SUP47
Summary: Enables translation release factor activity, codon specific. Involved in DNA-templated transcription termination; sno(s)RNA transcription; and translational termination. Located in cytoplasmic stress granule. Part of translation release factor complex. Orthologous to human ETF1 (eukaryotic translation termination factor 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SUP45 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (530869..532182, complement)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.
Title: Gene Amplification as a Mechanism of Yeast Adaptation to Nonsense Mutations in Release Factor Genes.
Transcriptome-wide investigation of stop codon readthrough in Saccharomyces cerevisiae.
Title: Transcriptome-wide investigation of stop codon readthrough in Saccharomyces cerevisiae.
Mechanisms that ensure speed and fidelity in eukaryotic translation termination.
Title: Mechanisms that ensure speed and fidelity in eukaryotic translation termination.
The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis.
Title: The catalytic activity of the translation termination factor methyltransferase Mtq2-Trm112 complex is required for large ribosomal subunit biogenesis.
Defects in the Dbp5-guided eRF1 delivery lead to premature contact and premature dissociation of eRF1 and eRF3 from the ribosome and to subsequent stop codon readthrough. Thus, the stepwise Dbp5-controlled termination complex assembly is essential for regular translation termination events
Title: Translation termination depends on the sequential ribosomal entry of eRF1 and eRF3.
The study used analytical ultracentrifugation with fluorescent detection system to identify the protein complexome of eRF1 in Saccharomyces cerevisiae. In addition to eRF1 presence in translating polysomes, the study found that eRF1 is associated with five other macromolecular complexes: 77S, 57S, 39S, 28S, and 20S in size.
Title: Defining the protein complexome of translation termination factor eRF1: Identification of four novel eRF1-containing complexes that range from 20S to 57S in size.
We demonstrated that interaction of [SWI+] and [PIN+] causes inactivation of SUP45 gene that leads to nonsense suppression. Our data show that prion interactions may cause heritable traits in Saccharomyces cerevisiae.
Title: Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae.
In this work, the authors show that nonsense and missense mutations in the SUP45, but not the SUP35, gene abolish diploid pseudohyphal and haploid invasive growth.
Title: The translation termination factor eRF1 (Sup45p) of Saccharomyces cerevisiae is required for pseudohyphal growth and invasion.
Analysis of the L123 systematic mutants of eRF1 suggested that this amino acid functions in stop codon discrimination in a manner coupled with eRF3 binding, and distinctive from previously reported adjacent residues.
Title: Genetic analysis of L123 of the tRNA-mimicking eukaryote release factor eRF1, an amino acid residue critical for discrimination of stop codons.
We characterized a region of the eRF1 N-terminal domain, the P1 pocket, that we had previously shown to be involved in termination efficiency. We identified two residues, arginine 65 and lysine 109, as critical for recognition of the three stop codons.
Title: New insights into stop codon recognition by eRF1.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables sequence-specific mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables translation release factor activity	IDA Inferred from Direct Assay more info	PubMed
enables translation release factor activity	IEA Inferred from Electronic Annotation more info
enables translation release factor activity, codon specific	IBA Inferred from Biological aspect of Ancestor more info
enables translation release factor activity, codon specific	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription termination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in cytoplasmic translational termination	IBA Inferred from Biological aspect of Ancestor more info
involved_in gene expression	IEA Inferred from Electronic Annotation more info
involved_in sno(s)RNA transcription	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational termination	IDA Inferred from Direct Assay more info	PubMed
involved_in translational termination	IEA Inferred from Electronic Annotation more info
involved_in translational termination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in translational termination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
part_of translation release factor complex	IBA Inferred from Biological aspect of Ancestor more info
part_of translation release factor complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: translation termination factor eRF1

NP_009701.3

Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

530869..532182 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178491.3 → NP_009701.3 TPA: translation termination factor eRF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009701.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VQD9, P12385

UniProtKB/TrEMBL

A6ZL86, B3LN14, B5VEA0, C7GY25, D3UEN8, G2W9E6, N1P6R7

Conserved Domains (1) summary

TIGR03676
Location:10 → 412

aRF1/eRF1; peptide chain release factor 1, archaeal and eukaryotic forms