ERG1 squalene monooxygenase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ERG1
Official Full Name: squalene monooxygenase
Primary source: SGD:S000003407
Locus tag: YGR175C
See related: AllianceGenome:SGD:S000003407; FungiDB:YGR175C; VEuPathDB:YGR175C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables squalene monooxygenase activity. Involved in ergosterol biosynthetic process. Located in endoplasmic reticulum and lipid droplet. Used to study cancer. Orthologous to human SQLE (squalene epoxidase). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See ERG1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (846933..848423, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Regulated targeting of the monotopic hairpin membrane protein Erg1 requires the GET pathway.
Title: Regulated targeting of the monotopic hairpin membrane protein Erg1 requires the GET pathway.
the spt23Delta mutant strain shows similar ERG1 expression to wild-type cells, while the mga2Delta/spt23Delta strain shows reduced ERG1 expression, comparable to the mga2Delta, suggesting that the role of regulation of ERG1 transcription is unique to Mga2p.
Title: A role for MGA2, but not SPT23, in activation of transcription of ERG1 in Saccharomyces cerevisiae.
gene down-regulation increases the levels of the sterol precursor squalene twofold upon treatment with hydroge peroxide
Title: Modulation of plasma membrane lipid profile and microdomains by H2O2 in Saccharomyces cerevisiae.
Saccharomyces cerevisiae squalene epoxidase contains two highly conserved motifs, 1 and 2, of unknown function. Amino acid substitutions in both regions reduce enzyme activity and/or alter allylamine sensitivity.
Title: Structure-function correlations of two highly conserved motifs in Saccharomyces cerevisiae squalene epoxidase.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info
enables squalene monooxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables squalene monooxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables squalene monooxygenase activity	IEA Inferred from Electronic Annotation more info
enables squalene monooxygenase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in ergosterol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ergosterol biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ergosterol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sterol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in sterol metabolic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
is_active_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in lipid droplet	IDA Inferred from Direct Assay more info	PubMed
located_in lipid droplet	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: squalene monooxygenase

NP_011691.1

EC 1.14.14.17

Squalene epoxidase; catalyzes epoxidation of squalene to 2,3-oxidosqualene; plays essential role in ergosterol-biosynthesis pathway and is specific target of antifungal drug terbinafine; directed to ER via guided entry of tail-anchored proteins (GET) pathway; degradation by ERAD triggered by membrane lipid saturation; human SQLE functionally complements the lethality of the erg1 null mutation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

846933..848423 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181304.1 → NP_011691.1 TPA: squalene monooxygenase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011691.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUW0, P32476

UniProtKB/TrEMBL

A6ZUI8, B3LI54, C7GT22, C8Z972, G2WEQ6, N1P3V6

Conserved Domains (3) summary

PRK06370
Location:19 → 51

PRK06370; FAD-containing oxidoreductase

pfam08491
Location:201 → 473

SE; Squalene epoxidase

cl21454
Location:19 → 86

NADB_Rossmann; Rossmann-fold NAD(P)(+)-binding proteins