RTT107 Rtt107p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RTT107
Official Full Name: Rtt107p
Primary source: SGD:S000001197
Locus tag: YHR154W
See related: AllianceGenome:SGD:S000001197; FungiDB:YHR154W; VEuPathDB:YHR154W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ESC4
Summary: Enables chromatin binding activity. Involved in several processes, including DNA double-strand break attachment to nuclear envelope; negative regulation of transposition, RNA-mediated; and regulation of DNA double-strand break processing. Located in cell periphery and nucleus. Part of Cul8-RING ubiquitin ligase complex. Orthologous to human PAXIP1 (PAX interacting protein 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See RTT107 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (402966..406178)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Functional studies show that Rtt107 binding of partner proteins of diverse activities promotes genome replication and stability in both distinct and concerted manners. A unified theme is that tetra- and di-BRCT domains of Rtt107 collaborate to recruit partner proteins to chromatin
Title: Molecular Basis for Control of Diverse Genome Stability Factors by the Multi-BRCT Scaffold Rtt107.
Rtt107 prevents checkpoint hyperactivation after replicative stress by limiting DNA damage
Title: Budding yeast Rtt107 prevents checkpoint hyperactivation after replicative stress by limiting DNA damage.
The scaffold protein Rtt107, which binds the Mus81-Mms4 complex, interacts with Cdc7 and thereby targets Cdc7-Dbf4 and Cdc5 to the complex enabling full Mus81 activation.
Title: Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis.
A key function of the Rtt107 BRCT domains for targeting of both itself and its interaction partners to DNA lesions was revealed.
Title: Rtt107 BRCT domains act as a targeting module in the DNA damage response.
Slx4 and Rtt107 control checkpoint signalling and DNA resection at double-strand breaks.
Title: Slx4 and Rtt107 control checkpoint signalling and DNA resection at double-strand breaks.
Rtt107 is a multi-functional scaffold supporting replication progression with partners SUMO, ubiquitin ligase E3, and SLX4.
Title: Rtt107 Is a Multi-functional Scaffold Supporting Replication Progression with Partner SUMO and Ubiquitin Ligases.
The DNA repair scaffold proteins Slx4 and Rtt107 utilize a minimal multi-BRCT-domain module for phosphatase-independent downregulation of DNA damage response signals in yeast.
Title: Dampening DNA damage checkpoint signalling via coordinated BRCT domain interactions.
The C-terminal tandem BRCT repeats of Rtt107 interaction with gammaH2A is functionally important during DNA repair.
Title: Structure of C-terminal tandem BRCT repeats of Rtt107 protein reveals critical role in interaction with phosphorylated histone H2A during DNA damage repair.
a multifaceted functional relationship between Rtt107 and Dot1 in the DNA damage response and maintenance of genome integrity.
Title: Loss of H3 K79 trimethylation leads to suppression of Rtt107-dependent DNA damage sensitivity through the translesion synthesis pathway.
Multiple fork repair factors associate with Rtt107 or Slx4, supporting that Mec1-dependent assembly of the Rtt107-Slx4-Dpb11 complex functions to coordinate fork repair.
Title: DNA damage signaling recruits the Rtt107-Slx4 scaffolds via Dpb11 to mediate replication stress response.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA double-strand break attachment to nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA double-strand break attachment to nuclear envelope	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA damage checkpoint	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrotransposon silencing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Cul8-RING ubiquitin ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Cul8-RING ubiquitin ligase complex	IDA Inferred from Direct Assay more info	PubMed
located_in cell periphery	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Rtt107p

NP_012024.1

BRCA1 C-terminal domain protein needed for recovery from DNA damage; implicated in Mms22-dependent DNA repair during S phase; involved in recruiting SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

402966..406178

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179285.1 → NP_012024.1 TPA: Rtt107p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012024.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLA3, P38850

UniProtKB/TrEMBL

G2WFM7, N1P2D3

Conserved Domains (5) summary

PTZ00121
Location:580 → 824

PTZ00121; MAEBL; Provisional

pfam00533
Location:117 → 213

BRCT; BRCA1 C-terminus (BRCT) domain

pfam16770
Location:830 → 914

RTT107_BRCT_5; Regulator of Ty1 transposition protein 107 BRCT domain

pfam16771
Location:948 → 1054

RTT107_BRCT_6; Regulator of Ty1 transposition protein 107 BRCT domain

cl00038
Location:382 → 456

BRCT; C-terminal domain of the breast cancer suppressor protein (BRCA1) and related domains