ASF1 nucleosome assembly factor ASF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ASF1
Official Full Name: nucleosome assembly factor ASF1
Primary source: SGD:S000003651
Locus tag: YJL115W
See related: AllianceGenome:SGD:S000003651; FungiDB:YJL115W; VEuPathDB:YJL115W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CIA1
Summary: Enables histone binding activity. Involved in several processes, including DNA replication-dependent chromatin assembly; chromatin remodeling; and positive regulation of transcription elongation by RNA polymerase II. Located in cytosol and nucleus. Orthologous to several human genes including ASF1A (anti-silencing function 1A histone chaperone). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See ASF1 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (196287..197126)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yeast Protein Asf1 Possesses Modulating Activity towards Protein Kinase CK2.
Title: Yeast Protein Asf1 Possesses Modulating Activity towards Protein Kinase CK2.
Data show that histone chaperone Anti-silencing factor 1 (Asf1) and H3K14 acetylation are both required for the efficient acetylation of H3K56.
Title: Two factor authentication: Asf1 mediates crosstalk between H3 K14 and K56 acetylation.
our study identified a novel mechanism by which H3K4me3 antagonizes the HIR/Asf1/Rtt106 repressor complex to promote histone gene expression and extend chronological life span.
Title: Set1-catalyzed H3K4 trimethylation antagonizes the HIR/Asf1/Rtt106 repressor complex to promote histone gene expression and chronological life span.
Data show that H3 histone acetyltransferase RTT109 (Rtt109) C-terminus interacts directly with nucleosome assembly factor ASF1 (Asf1) and the structural basis of this interaction.
Title: Structural characterization of the Asf1-Rtt109 interaction and its role in histone acetylation.
Data indicate that histone H2B ubiquitylation (H2Bub) and Asf1 cooperatively promote transcriptional silencing at telomeres and mating loci.
Title: H2B ubiquitylation and the histone chaperone Asf1 cooperatively mediate the formation and maintenance of heterochromatin silencing.
he model provides a molecular basis for the involvement of both Vps75 and Asf1 in Rtt109 catalysed histone H3 K9 acetylation. In the absence of Asf1 this model can be used to generate a complex consisting of a reconfigured Vps75 tetramer bound to a H3-H4 tetramer.
Title: The histone chaperone Vps75 forms multiple oligomeric assemblies capable of mediating exchange between histone H3-H4 tetramers and Asf1-H3-H4 complexes.
dissociation of histone H3 from Asf1 is required for efficient recovery and that Asf1 is required for complete dephosphorylation of Rad53 when the upstream DNA damage checkpoint signaling is turned off
Title: Asf1 facilitates dephosphorylation of Rad53 after DNA double-strand break repair.
Here, we find that Sas2-dependent acetylated histone H4 K16Ac in bulk histones requires passage of the cells through the S-phase of the cell cycle, and the rate of increase in H4 K16Ac depends on both CAF-I and Asf1.
Title: A link between Sas2-mediated H4 K16 acetylation, chromatin assembly in S-phase by CAF-I and Asf1, and nucleosome assembly by Spt6 during transcription.
Vps75 and Asf1 both enhance Rtt109 acetylation for H3/H4
Title: Utilizing targeted mass spectrometry to demonstrate Asf1-dependent increases in residue specificity for Rtt109-Vps75 mediated histone acetylation.
These results along with high association of Asf1 with heterochromatic centromeres and telomeres ascribe novel roles to Asf1 in condensin loading and chromatin dynamics.
Title: Genome-wide mapping of yeast histone chaperone anti-silencing function 1 reveals its role in condensin binding with chromatin.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables histone binding	IBA Inferred from Biological aspect of Ancestor more info
enables histone binding	IEA Inferred from Electronic Annotation more info
enables histone binding	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication-dependent chromatin assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication-dependent chromatin assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in chromatin organization	IDA Inferred from Direct Assay more info	PubMed
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in nucleosome assembly	IEA Inferred from Electronic Annotation more info
involved_in nucleosome disassembly	IEA Inferred from Electronic Annotation more info
involved_in nucleosome disassembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in subtelomeric heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in chromatin	IBA Inferred from Biological aspect of Ancestor more info
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: nucleosome assembly factor ASF1

NP_012420.1

Nucleosome assembly factor; involved in chromatin assembly, disassembly; required for recovery after DSB repair; role in H3K56 acetylation required for expression homeostasis, buffering mRNA synthesis rate against gene dosage changes in S phase; anti-silencing protein, derepresses silent loci when overexpressed; role in regulating Ty1 transposition; relocalizes to cytosol under hypoxia; growth defect of asf1 null is functionally complemented by either human ASF1A or ASF1B

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

196287..197126

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181548.1 → NP_012420.1 TPA: nucleosome assembly factor ASF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012420.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VW69, P32447

UniProtKB/TrEMBL

B3LQ02, B5VL60, C7GQ11, C8ZB51, G2WGQ6, N1P246

Conserved Domains (1) summary

pfam04729
Location:1 → 154

ASF1_hist_chap; ASF1 like histone chaperone