VAC14 Vac14p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: VAC14
Gene description: Vac14p
Primary source: FungiDB:YLR386W
Locus tag: YLR386W
See related: SGD:S000004378; AllianceGenome:SGD:S000004378; VEuPathDB:YLR386W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein-macromolecule adaptor activity. Involved in phosphatidylinositol biosynthetic process; positive regulation of lipid kinase activity; and protein localization to vacuolar membrane. Located in fungal-type vacuole membrane. Is extrinsic component of vacuolar membrane. Part of PAS complex. Human ortholog(s) of this gene implicated in striatonigral degeneration. Orthologous to human VAC14 (VAC14 component of PIKFYVE complex). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See VAC14 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (893628..896270)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and funct
Title: Vac14 protein multimerization is a prerequisite step for Fab1 protein complex assembly and function.
The authors show that Vac14 exerts an effect as a scaffold for the PI(3,5)P2 regulatory complex by direct contact with the known regulators of PI(3,5)P2: Fig4, Fab1, Vac7 and Atg18.
Title: VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and mouse.
Vac14p-Fig4p complex controls the hyperosmotic shock-induced increase in phosphatidylinositol 3,5-diphosphate levels.
Title: The Vac14p-Fig4p complex acts independently of Vac7p and couples PI3,5P2 synthesis and turnover.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein-macromolecule adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-macromolecule adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in cellular hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular hyperosmotic response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylinositol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol biosynthetic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in phosphatidylinositol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of phosphorylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to vacuolar membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of PAS complex	IBA Inferred from Biological aspect of Ancestor more info
part_of PAS complex	IDA Inferred from Direct Assay more info	PubMed
part_of PAS complex	IEA Inferred from Electronic Annotation more info
part_of PAS complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in endosome membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Vac14p

NP_013490.3

Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

893628..896270

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182275.3 → NP_013490.3 TPA: Vac14p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013490.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZ21, Q06708

UniProtKB/TrEMBL

A0A8H4BZY4, B5VNS3, C7GXJ0, C8ZDZ5, N1P7A8

Conserved Domains (3) summary

sd00044
Location:87 → 115

HEAT; HEAT repeat [structural motif]

pfam11916
Location:573 → 746

Vac14_Fig4_bd; Vacuolar protein 14 C-terminal Fig4p binding

pfam12755
Location:59 → 156

Vac14_Fab1_bd; Vacuolar 14 Fab1-binding region