SLX4 Slx4p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: SLX4
Gene description: Slx4p
Primary source: FungiDB:YLR135W
Locus tag: YLR135W
See related: SGD:S000004125; AllianceGenome:SGD:S000004125; VEuPathDB:YLR135W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 5'-flap endonuclease activity. Involved in several processes, including DNA metabolic process; regulation of DNA damage checkpoint; and regulation of DNA double-strand break processing. Located in nucleus. Part of Slx1-Slx4 complex. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SLX4 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (413281..415527)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structure specific DNA recognition by the SLX1-SLX4 endonuclease complex.
Title: Structure specific DNA recognition by the SLX1-SLX4 endonuclease complex.
SLX4 contributes to the appropriate distribution of crossovers and meiotic double-strand break formation on bivalents during meiosis.
Title: Budding Yeast SLX4 Contributes to the Appropriate Distribution of Crossovers and Meiotic Double-Strand Break Formation on Bivalents During Meiosis.
proper termination of DNA damage checkpoint signaling following the bypass of replication blocks imposed by alkylated DNA adducts requires the concerted and highly complementary actions of Slx4 and the PP4 phosphatase
Title: Termination of Replication Stress Signaling via Concerted Action of the Slx4 Scaffold and the PP4 Phosphatase.
Slx4 and Rtt107 control checkpoint signalling and DNA resection at double-strand breaks.
Title: Slx4 and Rtt107 control checkpoint signalling and DNA resection at double-strand breaks.
Rtt107 is a multi-functional scaffold supporting replication progression with partners SUMO, ubiquitin ligase E3, and SLX4.
Title: Rtt107 Is a Multi-functional Scaffold Supporting Replication Progression with Partner SUMO and Ubiquitin Ligases.
The Slx4-Dpb11 scaffold complex coordinates the response to replication fork stalling in S-phase and the subsequent mitosis.
Title: The Slx4-Dpb11 scaffold complex: coordinating the response to replication fork stalling in S-phase and the subsequent mitosis.
Authors demonstrate that Slx4 is recruited to chromatin behind stressed replication forks, in a region that is spatially distinct from that occupied by the replication machinery.
Title: Assembly of Slx4 signaling complexes behind DNA replication forks.
Multiple fork repair factors associate with Rtt107 or Slx4, supporting that Mec1-dependent assembly of the Rtt107-Slx4-Dpb11 complex functions to coordinate fork repair.
Title: DNA damage signaling recruits the Rtt107-Slx4 scaffolds via Dpb11 to mediate replication stress response.
Rtt107 binds to the DNA repair protein Slx4. Cells lacking Slx4 have similar phenotypes to those lacking Rtt107. Slx4 is required for the DNA damage dependent phosphorylation of Rtt107 by the checkpoint kinase Mec1.
Title: Slx4 regulates DNA damage checkpoint-dependent phosphorylation of the BRCT domain protein Rtt107/Esc4.
Slx4 is a known target of the DNA damage response that forms a complex with Rad1-Rad10 and is critical for 3'-end processing during repair of DSBs by single-strand annealing
Title: Mutants defective in Rad1-Rad10-Slx4 exhibit a unique pattern of viability during mating-type switching in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 5'-flap endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables 5'-flap endonuclease activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA-templated DNA replication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via break-induced replication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via single-strand annealing, removal of nonhomologous ends	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via single-strand annealing, removal of nonhomologous ends	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in interstrand cross-link repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of DNA damage checkpoint	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Slx1-Slx4 complex	IEA Inferred from Electronic Annotation more info
part_of Slx1-Slx4 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: Slx4p

NP_013236.1

Endonuclease involved in processing DNA; acts during recombination, repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in complex with Slx1p; involved in interstrand cross-link repair, Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress; FANCP-related factor

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

413281..415527

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182022.1 → NP_013236.1 TPA: Slx4p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013236.1

Status: REVIEWED

UniProtKB/Swiss-Prot

B3LT96, B5VN64, D6VYD0, Q12098

UniProtKB/TrEMBL

C7GMI7, C8ZDA4, G2WIX4, N1P6N1

Conserved Domains (2) summary

PLN02698
Location:585 → 692

PLN02698; Probable pectinesterase/pectinesterase inhibitor

pfam09494
Location:685 → 745

Slx4; Slx4 endonuclease