SPT10 Spt10p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SPT10
Official Full Name: Spt10p
Primary source: SGD:S000003663
Locus tag: YJL127C
See related: AllianceGenome:SGD:S000003663; FungiDB:YJL127C; VEuPathDB:YJL127C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CRE1; SUD1
Summary: Enables promoter-specific chromatin binding activity and sequence-specific DNA binding activity. Involved in DNA repair; negative regulation of autophagy; and negative regulation of macromolecule biosynthetic process. Located in nucleus. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See SPT10 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (182298..184220, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Diacylglycerol kinase alleviates autophagic degradation of the endoplasmic reticulum in SPT10-deficient yeast to enhance triterpene biosynthesis.
Title: Diacylglycerol kinase alleviates autophagic degradation of the endoplasmic reticulum in SPT10-deficient yeast to enhance triterpene biosynthesis.
Replication stress inhibits synthesis of histone mRNAs in yeast by removing Spt10p and Spt21p from the histone promoters.
Title: Replication stress inhibits synthesis of histone mRNAs in yeast by removing Spt10p and Spt21p from the histone promoters.
Spt10 is critical for normal cell division.
Title: Cell-cycle perturbations suppress the slow-growth defect of spt10Δ mutants in Saccharomyces cerevisiae.
suggest an interaction between Grx5 and SPT10 via intermolecular disulfide linkage and propose a model for a role of Grx5 in the regulation of protein expression under the control of SPT10
Title: Interaction between Saccharomyces cerevisiae glutaredoxin 5 and SPT10 and their in vivo functions.
Spt10 and Spt21 control silencing in S. cerevisiae by altering chromatin structure through roles beyond the control of histone gene expression.
Title: Spt10 and Spt21 are required for transcriptional silencing in Saccharomyces cerevisiae.
Spt10p is a dimer and that cooperative binding to the histone upstream activating sequences correlates with dimer formation, which is mediated through its N-terminal domain.
Title: Cooperative binding of the yeast Spt10p activator to the histone upstream activating sequences is mediated through an N-terminal dimerization domain.
Spt10p is a sequence-specific activator of the histone genes, possessing a DNA-binding domain fused to a likely histone acetyltransferase (HAT) domain.
Title: Global regulation by the yeast Spt10 protein is mediated through chromatin structure and the histone upstream activating sequence elements.
the C388S mutation disables the DNA-binding function of Spt10p in vitro and in vivo
Title: The DNA-binding domain of the yeast Spt10p activator includes a zinc finger that is homologous to foamy virus integrase.
SPT10, a putative acetyltransferase, is required for cell cycle-specific K56 acetylation at histone genes.
Title: Acetylation in histone H3 globular domain regulates gene expression in yeast.
mutations lethal when combined with spt21 mutations
Title: Evidence that Spt10 and Spt21 of Saccharomyces cerevisiae play distinct roles in vivo and functionally interact with MCB-binding factor, SCB-binding factor and Snf1.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables acyltransferase activity, transferring groups other than amino-acyl groups	IEA Inferred from Electronic Annotation more info
enables promoter-specific chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rDNA heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed

General protein information

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Preferred Names: Spt10p

NP_012408.1

Histone H3 acetylase with a role in transcriptional regulation; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near TATA box; involved in S phase-specific acetylation of H3K56 at histone promoters, which is required for recruitment of SWI/SNF nucleosome remodeling complex and subsequent transcription

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

182298..184220 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181560.1 → NP_012408.1 TPA: Spt10p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012408.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VW59, P35208

UniProtKB/TrEMBL

A6ZQK4, B3LPZ2, B5VL52, C8ZB92

Conserved Domains (2) summary

pfam09337
Location:351 → 389

zf-H2C2; His(2)-Cys(2) zinc finger

cl17182
Location:167 → 256

NAT_SF; N-Acyltransferase superfamily: Various enyzmes that characteristicly catalyze the transfer of an acyl group to a substrate