DED1 DEAD-box ATP-dependent RNA helicase DED1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DED1
Official Full Name: DEAD-box ATP-dependent RNA helicase DED1
Primary source: SGD:S000005730
Locus tag: YOR204W
See related: AllianceGenome:SGD:S000005730; FungiDB:YOR204W; VEuPathDB:YOR204W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SPP81
Summary: Enables RNA binding activity; RNA helicase activity; and eukaryotic initiation factor 4G binding activity. Involved in positive regulation of formation of translation preinitiation complex; spliceosomal complex disassembly; and translational initiation. Located in cytoplasmic stress granule and nucleus. Used to study childhood medulloblastoma. Human ortholog(s) of this gene implicated in colorectal cancer and syndromic X-linked intellectual disability. Orthologous to human DDX3X (DEAD-box helicase 3 X-linked) and DDX3Y (DEAD-box helicase 3 Y-linked). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See DED1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (722911..724725)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Contribution of the yeast bi-chaperone system in the restoration of the RNA helicase Ded1 and translational activity under severe ethanol stress.
Title: Contribution of the yeast bi-chaperone system in the restoration of the RNA helicase Ded1 and translational activity under severe ethanol stress.
The DEAD-Box RNA Helicase Ded1 Is Associated with Translating Ribosomes.
Title: The DEAD-Box RNA Helicase Ded1 Is Associated with Translating Ribosomes.
A synthetic genetic array screen for interactions with the RNA helicase DED1 during cell stress in budding yeast.
Title: A synthetic genetic array screen for interactions with the RNA helicase DED1 during cell stress in budding yeast.
G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p.
Title: G-quadruplex DNA inhibits unwinding activity but promotes liquid-liquid phase separation by the DEAD-box helicase Ded1p.
Medulloblastoma-associated mutations in the DEAD-box RNA helicase DDX3X/DED1 cause specific defects in translation.
Title: Medulloblastoma-associated mutations in the DEAD-box RNA helicase DDX3X/DED1 cause specific defects in translation.
The In Silico Identification of Potential Members of the Ded1/DDX3 Subfamily of DEAD-Box RNA Helicases from the Protozoan Parasite Leishmania infantum and Their Analyses in Yeast.
Title: The In Silico Identification of Potential Members of the Ded1/DDX3 Subfamily of DEAD-Box RNA Helicases from the Protozoan Parasite Leishmania infantum and Their Analyses in Yeast.
Distinct interactions of eIF4A and eIF4E with RNA helicase Ded1 stimulate translation in vivo.
Title: Distinct interactions of eIF4A and eIF4E with RNA helicase Ded1 stimulate translation in vivo.
Results are consistent with a model wherein Ded1 stalls translation and specifically removes eIF4G1 from translation preinitiation complexes, thus removing eIF4G1 from the translating mRNA pool and leading to the codegradation of both proteins.
Title: The DEAD-box RNA helicase Ded1 has a role in the translational response to TORC1 inhibition.
Functional interplay between Ded1 and Dbp1 in preinitiation complex attachment and scanning on structured mRNAs in Saccharomyces cerevisiae has been reported.
Title: Functional interplay between DEAD-box RNA helicases Ded1 and Dbp1 in preinitiation complex attachment and scanning on structured mRNAs in vivo.
We propose a model where ATP-bound Ded1 stabilizes partially unwound duplexes and where multiple binding events may be needed to see displacement
Title: Mechanistic characterization of the DEAD-box RNA helicase Ded1 from yeast as revealed by a novel technique using single-molecule magnetic tweezers.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables RNA helicase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables RNA strand annealing activity	IDA Inferred from Direct Assay more info	PubMed
enables eukaryotic initiation factor 4G binding	IPI Inferred from Physical Interaction more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables translation initiation factor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in positive regulation of formation of translation preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of formation of translation preinitiation complex	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spliceosomal complex disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DEAD-box ATP-dependent RNA helicase DED1

NP_014847.3

EC 3.6.4.13

ATP-dependent DEAD-box RNA helicase with strand-annealing activity; promotes eIF4F-dependent 48S translation preinitiation complex (PIC) assembly, stimulating recruitment of mRNAs with long, structured 5'-UTRs; cooperates with Dbp1p in PIC attachment and scanning; ATPase activity stimulated by mRNA cap-associated factor binding; directly binds eIF4G; role in spliceosomal complex disassembly; mutation in human homolog DBY associated with male infertility; human homolog DDX3X complements the null

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

722911..724725

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183623.3 → NP_014847.3 TPA: DEAD-box ATP-dependent RNA helicase DED1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014847.3

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZP47, D6W2R0, P06634

UniProtKB/TrEMBL

B3LJM9, B5VS86, C7GWH8

Conserved Domains (2) summary

cd18787
Location:374 → 503

SF2_C_DEAD; C-terminal helicase domain of the DEAD box helicases

cd17967
Location:144 → 368

DEADc_DDX3_DDX4; DEAD-box helicase domain of ATP-dependent RNA helicases DDX3 and DDX4