HSF1 stress-responsive transcription factor HSF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: HSF1
Gene description: stress-responsive transcription factor HSF1
Primary source: FungiDB:YGL073W
Locus tag: YGL073W
See related: SGD:S000003041; AllianceGenome:SGD:S000003041; VEuPathDB:YGL073W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: EXA3; MAS3
Summary: Enables DNA-binding transcription factor activity. Involved in several processes, including negative regulation of TORC1 signaling; regulation of cellular macromolecule biosynthetic process; and regulation of establishment of protein localization to chromosome. Located in nucleus. Human ortholog(s) of this gene implicated in Huntington's disease; cataract; and cataract 5 multiple types. Orthologous to several human genes including HSF2 (heat shock transcription factor 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See HSF1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (368753..371254)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress.
Title: Deletion of the transcription factors Hsf1, Msn2 and Msn4 in yeast uncovers transcriptional reprogramming in response to proteotoxic stress.
Cytoplasmic redox imbalance in the thioredoxin system activates Hsf1 and results in hyperaccumulation of the sequestrase Hsp42 with misfolded proteins.
Title: Cytoplasmic redox imbalance in the thioredoxin system activates Hsf1 and results in hyperaccumulation of the sequestrase Hsp42 with misfolded proteins.
Genetic inactivation of essential HSF1 reveals an isolated transcriptional stress response selectively induced by protein misfolding.
Title: Genetic inactivation of essential HSF1 reveals an isolated transcriptional stress response selectively induced by protein misfolding.
Oxidation of two cysteines within yeast Hsp70 impairs proteostasis while directly triggering an Hsf1-dependent cytoprotective response.
Title: Oxidation of two cysteines within yeast Hsp70 impairs proteostasis while directly triggering an Hsf1-dependent cytoprotective response.
Hsf1 activation by proteotoxic stress requires concurrent protein synthesis.
Title: Hsf1 activation by proteotoxic stress requires concurrent protein synthesis.
Cell adaptation to aneuploidy by the environmental stress response dampens induction of the cytosolic unfolded-protein response.
Title: Cell adaptation to aneuploidy by the environmental stress response dampens induction of the cytosolic unfolded-protein response.
Transient intracellular acidification regulates the core transcriptional heat shock response.
Title: Transient intracellular acidification regulates the core transcriptional heat shock response.
Hsp70 binds Hsf1 via its canonical substrate binding domain and Hsp70 regulates Hsf1 DNA-binding activity. During heat shock, Hsp70 is out-titrated by misfolded proteins derived from ongoing translation in the cytosol.
Title: Cytoplasmic protein misfolding titrates Hsp70 to activate nuclear Hsf1.
Authors show that Hsf1 phosphorylation promotes cell-to-cell variation, and this variation, rather than absolute Hsf1 activity, promotes antifungal resistance. Authors propose that Hsf1 phosphorylation enables differential tuning of the proteostasis network in individual cells, allowing populations to access a range of phenotypic states.
Title: Hsf1 Phosphorylation Generates Cell-to-Cell Variation in Hsp90 Levels and Promotes Phenotypic Plasticity.
The authors mapped a discrete Hsp70 binding site on Hsf1 to a C-terminal segment known as conserved element 2 (CE2). In vitro, CE2 binds to Hsp70 with low affinity (9 microM), in agreement with model requirements. In cells, removal of CE2 resulted in increased basal Hsf1 activity and delayed deactivation during heat shock, while tandem repeats of CE2 sped up Hsf1 deactivation.
Title: Hsf1 and Hsp70 constitute a two-component feedback loop that regulates the yeast heat shock response.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables sequence-specific DNA binding	HDA more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to heat	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of TORC1 signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of establishment of protein localization to chromosome	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of translation in response to stress	IDA Inferred from Direct Assay more info	PubMed
involved_in response to heat	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrion	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: stress-responsive transcription factor HSF1

NP_011442.3

Trimeric heat shock transcription factor; forms condensate with Mediator and RNA Pol II upon heat shock; activates multiple genes in response to diverse stresses and transient intracellular acidification; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status through RQC (Ribosomal Quality Control)-mediated translation-stress signal; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

368753..371254

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180938.3 → NP_011442.3 TPA: stress-responsive transcription factor HSF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011442.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU70, P10961, P11529

UniProtKB/TrEMBL

C7GW40, N1P1W2

Conserved Domains (1) summary

COG5169
Location:165 → 453

HSF1; Heat shock transcription factor [Transcription]