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GTR Home > Tests > Monogenic Kidney Stone Panel

Overview

Test name

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Monogenic Kidney Stone Panel (Monogenic NGS panel)

Purpose of the test

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Condition

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Methodology

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Molecular Genetics
CSequence analysis of the entire coding region
Next-Generation (NGS)/Massively parallel sequencing (MPS)
  • Agilent 2100 Bioanalyzer
  • Agilent SureSelect
  • Covaris S2 Sonicator

Summary of what is tested

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Description of study

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We have developed a next generation sequencing (NGS) panel of ~160 genes associated or as candidates for kidney stone disease. Gene capture is performed using the NEBNext Ultra DNA Library Preparation kit (Illumina) in an automated way on a Bravo workstation and pooled libraries are then sequenced on a NovaSeq SP. Sequencing results are then analyzed by SVS pipeline (Golden Helix).

Researchers

Person responsible for the studyHelpPeter Harris, Lab DirectorPeter Harris, PhD, Lab Director
Study contactHelpPeter Harris, Lab Director, Harris.Peter@mayo.edu, 507-266-0541 (phone)Peter Harris, PhD, Lab Director, Harris.Peter@mayo.edu

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