Chromosome Walking

A technique with which an unknown region of a chromosome can be explored. It is generally used to isolate a locus of interest for which no probe is available but that is known to be linked to a gene which has been identified and cloned. A fragment containing a known gene is selected and used as a probe to identify other overlapping fragments which contain the same gene. The nucleotide sequences of these fragments can then be characterized. This process continues for the length of the chromosome.

Year introduced: 1991

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Tree Number(s): E05.393.183.250

MeSH Unique ID: D016386

Entry Terms:

• Chromosome Walkings
• Walking, Chromosome
• Walkings, Chromosome

Previous Indexing:

• Chromosome Mapping (1983-1990)
• Cloning, Molecular (1983-1990)

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Genetic Techniques
Chromosome Mapping
Chromosome Walking