In Situ Nick-End Labeling

An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.

Year introduced: 1999

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Tree Number(s): E05.393.475

MeSH Unique ID: D020287

Entry Terms:

• In Situ Nick End Labelling
• Nick-End Labelling, In Situ
• TUNEL
• In Situ Nick End-Labeling
• In Situ Nick End-Labelling
• In Situ Nick-End Labelling
• Nick End-Labeling, In Situ
• Nick End Labeling, In Situ
• Nick End-Labelling, In Situ
• Nick End Labelling, In Situ
• Nick-End Labeling, In Situ
• In Situ Nick End Labeling

Previous Indexing:

• Apoptosis (1994-1998)

See Also:

• Apoptosis
• Comet Assay

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Genetic Techniques
In Situ Nick-End Labeling