Nuclease Protection Assays

Techniques for measuring specific nucleic acid interaction with another nucleic acid or with a protein by digestion of the non-interacting nucleic acid by various nucleases. After all non-interacting regions are eliminated by nuclease digestion, the protected nucleic acid that remains is analyzed. DNA FOOTPRINTING utilizes this technique to analyze the DNA contact sites of DNA-BINDING PROTEINS.

Year introduced: 2001

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Tree Number(s): E05.393.600

MeSH Unique ID: D023282

Entry Terms:

• Assay, Nuclease Protection
• Assays, Nuclease Protection
• Nuclease Protection Assay

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Genetic Techniques
Nuclease Protection Assays