Microscopy, Fluorescence, Multiphoton
Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged.
Year introduced: 2003
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Tree Number(s): E01.370.350.515.458.500, E01.370.350.515.717.250, E05.595.458.500, E05.595.717.250
MeSH Unique ID: D036641
Entry Terms:
- Multiphoton Excitation Microscopy
- Excitation Microscopies, Multiphoton
- Excitation Microscopy, Multiphoton
- Microscopies, Multiphoton Excitation
- Microscopy, Multiphoton Excitation
- Multiphoton Excitation Microscopies
- Multiphoton Fluorescence Microscopy
- Microscopy, Multiphoton Fluorescence
- Fluorescence Microscopy, Multiphoton
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