Optical Restriction Mapping

A technique to generate restriction maps from single large DNA molecules by spreading the DNA onto a glass surface, digesting with DNA RESTRICTION ENZYMES, staining with FLUORESCENT DYES, and visualizing the DNA cleavage sites by FLUORESCENCE MICROSCOPY.

Year introduced: 2010

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Tree Number(s): E05.393.183.620.650.500, E05.393.712.500

MeSH Unique ID: D056972

Entry Terms:

• Restriction Mapping, Optical

Previous Indexing:

• Restriction Mapping (1993-2009)

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Genetic Techniques
Chromosome Mapping
Physical Chromosome Mapping
Restriction Mapping
Optical Restriction Mapping

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Genetic Techniques
Restriction Mapping
Optical Restriction Mapping