Alternative titles; symbols
HGNC Approved Gene Symbol: NUP43
Cytogenetic location: 6q25.1 Genomic coordinates (GRCh38): 6:149,724,315-149,749,358 (from NCBI)
Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP43 is part of one such subcomplex, NUP107 (607617)-NUP160 (607614) (Loiodice et al., 2004).
Cronshaw et al. (2002) identified NUP43 as a constituent of a purified nucleoporin fraction from rat liver nuclei. NUP43 contains WD repeats and has a calculated molecular mass of about 42 kD. Fluorescence-tagged NUP43, transfected into HeLa cells, showed punctate nuclear rim localization typical of nucleoporins.
By cotransfection and immunoprecipitation of HeLa cells, Loiodice et al. (2004) showed that NUP43 and several other WD-repeat nucleoporins are constituents of the Nup107-160 complex. All constituents of this complex were targeted to kinetochores from prophase to anaphase during mitosis.
Zuccolo et al. (2007) stated that the NUP107-NUP160 subcomplex contains NUP133 (607613), NUP96 (601021), NUP85 (170285), NUP43, NUP37 (609264), SEC13 (SEC13L1; 600152), and SEH1 (SEH1L; 609263). The NUP107-NUP160 subcomplex stably associates on both faces of NPCs during interphase, and the entire subcomplex is recruited to chromatin during mitosis. A fraction of the subcomplex localizes at kinetochores during prophase, even before nuclear envelope breakdown. Zuccolo et al. (2007) found that recruitment of the NUP107-NUP160 complex to kinetochores depended mainly on the NDC80 complex (see 607272) and CENPF (600236). The SEH1 subunit of the NUP107-NUP160 complex was essential for targeting the complex to kinetochores. Codepletion of several NUP107-NUP160 subunits or of SEH1 alone resulted in kinetochores that failed to establish proper microtubule attachment, thus inducing a checkpoint-dependent mitotic delay. The mitotic Ran-GTP effector, CRM1 (XPO1; 602559), as well as its binding partner, the RANGAP1 (602362)-RANBP2 (601181) complex, were mislocalized upon depletion of NUP107-NUP160 complex from kinetochores.
Hartz (2015) mapped the NUP43 gene to chromosome 6q25.1 based on an alignment of the NUP43 sequence (GenBank AF514997) with the genomic sequence (GRCh38).
Cronshaw, J. M., Krutchinsky, A. N., Zhang, W., Chait, B. T., Matunis, M. J. Proteomic analysis of the mammalian nuclear pore complex. J. Cell Biol. 158: 915-927, 2002. [PubMed: 12196509] [Full Text: https://doi.org/10.1083/jcb.200206106]
Hartz, P. A. Personal Communication. Baltimore, Md. 12/7/2015.
Loiodice, I., Alves, A., Rabut, G., van Overbeek, M., Ellenberg, J., Sibarita, J.-B., Doye, V. The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis. Molec. Biol. Cell 15: 3333-3344, 2004. [PubMed: 15146057] [Full Text: https://doi.org/10.1091/mbc.e03-12-0878]
Zuccolo, M., Alves, A., Galy, V., Bolhy, S., Formstecher, E., Racine, V., Sibarita, J.-B., Fukagawa, T., Shiekhattar, R., Yen, T., Doye, V. The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions. EMBO J. 26: 1853-1864, 2007. [PubMed: 17363900] [Full Text: https://doi.org/10.1038/sj.emboj.7601642]