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Inhibition of calcium stimulated Mycobacterium tuberculosis H37Ra CtpF mutant ATPase activity using Na2ATP as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by colorimetric analysis
Assay data:7 Tested
SummaryPubMed Citation
Inhibition of calcium stimulated Mycobacterium tuberculosis H37Ra CtpF ATPase activity using Na2ATP as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by colorimetric analysis
Assay data:5 Active, 7 Tested
SummaryCompounds, ActivePubMed Citation
Inhibition of Mycobacterium tuberculosis H37Ra ATCC 25177 adenosine kinase using varying levels of [3H]Ado as substrate in presence of adenosine deaminase inhibitor deoxycoformycin by Line-weaver burk plot analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Mycobacterium tuberculosis H37Ra PTPB using p-nitrophenyl phosphate as substrate
Assay data:1 Active, 1 Tested
Inhibition of Mycobacterium tuberculosis H37Ra PTPB expressed in Escherichia coli BL21 (DE3) using p-nitrophenyl phosphate as substrate preincubated for 10 mins followed by substrate addition and measured for 5 mins by spectrophotometric analysis
Assay data:24 Tested
Inhibition of Mycobacterium tuberculosis H37Ra PTPB expressed in Escherichia coli BL21 (DE3) using p-nitrophenyl phosphate as substrate preincubated for 10 mins followed by substrate addition measured for 5 mins by spectrophotometric analysis
Assay data:2 Tested
Inhibition of Mycobacterium tuberculosis H37Ra menG assessed as inhibition of 2-methyl-3-(3,7,11-trimethyldodeca-2,6,10-trienyl)naphthalene-1,4-dione formation using 2-(3,7,11-trimethyldodeca-2,6,10-trienyl)naphthalene-1,4-dione as substrate incubated for 3 hrs in presence of SAM by HPLC analysis
Assay data:6 Tested
Inhibition of InhA in Mycobacterium tuberculosis H37Ra assessed as decrease in trehalose dimycolates level at 200 uM incubated for 48 hrs using [14C]acetate by TLC based metabolic labeling assay relative to control
Assay data:1 Active, 6 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of InhA in Mycobacterium tuberculosis H37Ra assessed as accumulation of trehalose monomycolates at 200 uM incubated for 48 hrs using [14C]acetate by TLC based metabolic labeling assay relative to control
Inhibition of Mycobacterium tuberculosis H37Ra PTPB expressed in Escherichia coli BL21 (DE3) at 50 uM using p-nitrophenyl phosphate as substrate preincubated for 10 mins followed by substrate addition measured for 5 mins by spectrophotometric analysis
Assay data:12 Tested
Inhibition of DprE1 in Mycobacterium tuberculosis H37Ra assessed as accumulation of trehalose dimycolate at 0.5 ug/ml preincubated for 24 hrs followed by radiolabeling with [14C]-acetate for 24 hrs by TLC/autoradiographic method
Inhibition of DprE1 in Mycobacterium tuberculosis H37Ra assessed as accumulation of trehalose dimycolate at 0.2 ug/ml preincubated for 24 hrs followed by radiolabeling with [14C]-acetate for 24 hrs by TLC/autoradiographic method
Assay data:1 Active, 3 Tested
Inhibition of calcium stimulated Mycobacterium tuberculosis H37Ra CtpF ATPase activity at 100 uM using Na2ATP as substrate preincubated for 30 mins followed by substrate addition and measured after 30 mins by colorimetric analysis relative to control
Assay data:3 Tested
Inhibition of Mycobacterium tuberculosis HupB binding to fdxA promoter DNA at 500 uM by EMSA
Assay data:1 Tested
Inhibition of InhA in Mycobacterium tuberculosis H37Ra assessed as reduction in mycolic acids synthesis by measuring MAME level at 200 uM incubated for 48 hrs using [14C]acetate by TLC based metabolic labeling assay relative to control
Assay data:1 Active, 7 Tested
Inhibition of InhA in Mycobacterium tuberculosis H37Ra assessed as reduction in mycolic acids synthesis by measuring FAME level at 200 uM incubated for 48 hrs using [14C]acetate by TLC based metabolic labeling assay relative to control
Antitubercular activity against Mycobacterium tuberculosis H37Ra ATCC 25177 dormant stage measured on day 12 by XTT reduction menadione based two-fold dilution assay
Assay data:15 Tested
Antitubercular activity against Mycobacterium tuberculosis H37Ra ATCC 25177 active stage measured on day 8 by XTT reduction menadione based two-fold dilution assay
Antitubercular activity against Mycobacterium tuberculosis H37Ra ATCC 25177 active stage infected in human THP1 cells measured on day 8 by nitrate reductase assay
Antitubercular activity against Mycobacterium tuberculosis H37Ra ATCC 25177 dormant stage infected in human THP1 cells measured on day 12 by nitrate reductase assay
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