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Antioomycete activity against Phytophthora sojae P6497 after 48 to 96 hrs by disc diffusion method
Assay data:2 Tested
SummaryPubMed Citation
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as quenching rate constant at 27 degC by fluorescence spectroscopy
Assay data:1 Tested
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as quenching rate constant at 37 degC by fluorescence spectroscopy
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as quenching rate constant at 47 degC by fluorescence spectroscopy
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as equilibrium binding constant by fluorescence spectroscopy
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as positive entropy and enthalpy changes by themodynamic analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed Citation
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as static fluorescence quenching by FRET analysis
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as increase in alpha helicity of the protein at protein to compound ratio of 1:1 by CD spectroscopy (Rvb = 18.3%)
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as increase in alpha helicity of the protein at protein to compound ratio of 1:5 by CD spectroscopy (Rvb = 18.3%)
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as decrease in beta sheet content of the protein at protein to compound ratio of 1:1 by CD spectroscopy (Rvb = 25.6%)
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as decrease in beta sheet content of the protein at protein to compound ratio of 1:5 by CD spectroscopy (Rvb = 25.6%)
Inhibition of C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as reduction in initial velocity at the step of threonine activation preincubated for 10 min with 4 uM enzyme before threonine addition measured after 30 min by HPLC analysis
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) at 20 to 200 uM at 27 to 47 degC after 30 min by fluorescence quenching assay
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as Stern-Volmer quenching constant at 27 degC by fluorescence spectroscopy
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as Stern-Volmer quenching constant at 37 degC by fluorescence spectroscopy
Binding affinity to C-terminal His6-tagged Phytophthora sojae race 1 recombinant ThrRS expressed in Escherichia coli BL21 (Codonplus DE3) assessed as Stern-Volmer quenching constant at 47 degC by fluorescence spectroscopy
Antifungal activity against Phytophthora sojae race 1 assessed as radial growth inhibition at 0.006 ug/ml incubated at 28 degC in the dark for 6 to 8 days in presence of 0.6 mM threonine
Antifungal activity against Phytophthora sojae race 1 assessed as radial growth inhibition at 0.008 ug/ml incubated at 28 degC in the dark for 6 to 8 days in presence of 0.2 to 0.6 mM alanine
Antifungal activity against Phytophthora sojae race 1 assessed as radial growth inhibition at 0.008 ug/ml incubated at 28 degC in the dark for 6 to 8 days in presence of 0.2 to 0.6 mM glutamine
Antifungal activity against Phytophthora sojae race 1 assessed as radial growth inhibition at 0.008 ug/ml incubated at 28 degC in the dark for 6 to 8 days in presence of 0.2 to 0.6 mM tyrosine
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