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ATPase enzyme assay from Article 10.1021/acschembio.7b00041: "Discovery and Characterization of a Eukaryotic Initiation Factor 4A-3-Selective Inhibitor That Suppresses Nonsense-Mediated mRNA Decay."
Assay data:2 Active, 1 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of eIF4A (unknown origin) assessed as inhibition of translation of RNA featuring short 5'-UTR of tubulin
Assay data:5 Active, 5 Activity ≤ 1 µM, 5 Tested
Inhibition of eIF4A (unknown origin) assessed as inhibition of translation of RNA featuring highly structured 5'-UTR of c-Myc
Inhibition of eIF4A1 in human MDA-MB-231 cells assessed as reduction in BCL2 protein expression at 10 to 100 nM incubated for 24 hrs by Western blot analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of eIF4A1 in human MDA-MB-231 cells assessed as reduction in cyclin D1 protein expression at 10 to 100 nM incubated for 24 hrs by Western blot analysis
Inhibition of eIF4A1 in human HAP1 cells assessed as reduction in cell proliferation incubated for 72 hrs by CellTiter-Glo reagent based assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of eIF4A1 in human HAP1 cells assessed as reduction in Cyclin D1 protein expression at 5 to 50 nM incubated for 24 hrs by Western blot analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of eIF4A1 in human HAP1 cells assessed as reduction in MCL-1 protein expression at 5 to 50 nM incubated for 24 hrs by Western blot analysis
Inhibition of eIF4A1 in human HAP1 cells assessed as reduction in c-MYC protein expression at 5 to 50 nM incubated for 24 hrs by Western blot analysis
Inhibition of eIF4A1 in human MDA-MB-231 cells assessed as inhibition of cellular-translation incubated for 4 hrs by specific tandem sequence motif repeat CAACAA in 5'- UTR containing luciferase reporter gene assay
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Inhibition of eIF4A1 in human MDA-MB-231 cells assessed as inhibition of cellular-translation incubated for 4 hrs by specific tandem sequence motif repeat CCGCCG in 5'- UTR containing luciferase reporter gene assay
Inhibition of eIF4A1 in human MDA-MB-231 cells assessed as inhibition of cellular-translation incubated for 4 hrs by specific tandem sequence motif repeat GGCGGC in 5'- UTR containing luciferase reporter gene assay
Inhibition of eIF4A1 in human MDA-MB-231 cells assessed as inhibition of cellular-translation incubated for 4 hrs by specific tandem sequence motif repeat AGAGAG in 5'- UTR containing luciferase reporter gene assay
Ratio of half life for binding affinity to full length recombinant eIF4A1 (unknown origin) assessed as induction ternary complex formation in presence of AGAGAG by measuring half life for AGAGAG + AMP-PNP complex formation SPR assay to half life for binding affinity to full length recombinant eIF4A1 (unknown origin) assessed as induction ternary complex formation in presence of AGAGAG by measuring half life for AGAGAG + ATP complex formation SPR assay
Binding affinity to full length recombinant eIF4A1 (unknown origin) assessed as induction ternary complex formation in presence of DNA oligo sequences by measuring Kd for DNA oligo sequences + ATP complex formation SPR assay
Binding affinity to full length recombinant eIF4A1 (unknown origin) assessed as induction ternary complex formation in presence of AGAGAG by measuring half life for AGAGAG + AMP-PNP complex formation SPR assay
Binding affinity to full length recombinant eIF4A1 (unknown origin) assessed as induction ternary complex formation in presence of AGAGAG by measuring half life for AGAGAG + ATP complex formation SPR assay
Binding affinity to full length recombinant eIF4A1 (unknown origin) assessed as induction ternary complex formation in presence of AGAGAG by measuring Kd for CAACAA + ATP complex formation SPR assay (Rvb = 3.78 +/- 0.05 microM)
Binding affinity to full length recombinant eIF4A1 (unknown origin) assessed as induction ternary complex formation in presence of AGAGAG by measuring Kd for CCGCCG + ATP complex formation SPR assay (Rvb = 3.27 +/- 0.06 microM)
Binding affinity to full length recombinant eIF4A1 (unknown origin) assessed as induction ternary complex formation in presence of AGAGAG by measuring Kd for GGCGGC + ATP complex formation SPR assay (Rvb = 8 +/- 0.3 microM)
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