Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Potency index, ratio of 7-(diethylamino)-4-methyl-2H-chromen-2-one EC50 to test compound EC50 for stabilization of AL patient derived full length fluorescein-labeled IGLV6-57 expressed in Escherichia coli BL21 (DE3) measured after 24 hrs by proteolysis coupled-fluorescence polarization assay
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Stabilization of AL patient derived fluorescein-labeled IGLV6-57 T46L/F49Y mutant in 93% human plasma assessed as protection against proteinase K-mediated digestion at 10 uM after 2 hrs by SDS-PAGE method
Assay data:2 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Stabilization of AL patient derived IGLV6-57 F98D mutant assessed as fold protection against proteinase K-mediated digestion
Assay data:3 Tested
Stabilization of AL patient derived full length fluorescein-labeled IGLV6-57 expressed in Escherichia coli BL21 (DE3) measured after 24 hrs by proteolysis coupled-fluorescence polarization assay
Assay data:38 Active, 24 Activity ≤ 1 µM, 42 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Stabilization of AL patient derived fluorescein-labeled IGLV6-57 T46L/F49Y mutant measured after 24 hrs by proteolysis coupled-fluorescence polarization assay
Assay data:35 Active, 23 Activity ≤ 1 µM, 37 Tested
Binding affinity to AL patient derived full length IGLV6-57 assessed as protection against proteinase K-mediated digestion by measuring fraction remaining at 100 uM measured after 2 hrs by analytical size-exclusion chromatography
Kinetic stabilization of AL patient derived full length IGLV6-57 assessed as interconversion between bound and unbound light chains by NMR based CEST analysis
Assay data:1 Tested
Kinetic stabilization of AL patient derived full length IGLV6-57 assessed as conformational exchange between bound and unbound light chains by measuring signal at 100 uM by [15N-1H]HSQC NMR spectra analysis
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on