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GSK3B SGC Tm panel (DSF)
Assay data:1 Tested
SummaryRelated BioAssays by Target
GSK3B Invitrogen Lab (UK) kinase screen (BI)
Inhibition of GSK3beta (unknown origin) at 10 uM by biochemical hotspot kinase assay relative to control
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of GSK3beta (unknown origin) at 1 uM relative to control
Assay data:3 Tested
Inhibition of recombinant human GSK3beta (636 to 661 residues) assessed as reduction in human muscle glycogen synthase type 1 fragment phosphorylation using YRRAAVPPSPSLSRHSSPHQ(pS)EDE as substrate in presence of ATP incubated for 60 mins by ADP-Glo kinase assay
Assay data:11 Active, 5 Activity ≤ 1 µM, 20 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of recombinant human GSK3beta (636 to 661 residues) assessed as reduction in human muscle glycogen synthase type 1 fragment phosphorylation using YRRAAVPPSPSLSRHSSPHQ(pS)EDE as substrate in presence of ATP at 100 uM incubated for 60 mins by ADP-Glo kinase assay
Assay data:20 Tested
Inhibition of GSK-3beta (unknown origin)
Assay data:11 Active, 11 Activity ≤ 1 µM, 12 Tested
Inhibition of GSK3beta phosphorylation in human HT-29 cells at 4 uM incubated for 72 hrs by Western blot analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of GSK3beta phosphorylation in human HT-29 cells at 2 uM incubated for 72 hrs by Western blot analysis
Inhibition of GSK3beta phosphorylation in human HT-29 cells at 1 uM incubated for 72 hrs by Western blot analysis
Inhibition of GSK3beta (unknown origin) assessed as inhibition of substrate peptide phosphorylation using eIF4E-binding protein 1 as peptide substrate incubated for 1 hr in presence of ATP by Lance Ultra HTRF assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Non-competitive inhibition of GSK3-beta (unknown origin) assessed as inhibition of tau hyperphosphorylation at 100 ug/ml incubated for 1 hr in presence of ATP by ADP-Glo kinase assay relative to control
Assay data:2 Tested
Inhibition of human recombinant full-length GSK3beta expressed in Escherichia coli incubated for 2 hrs
Inhibition of GSK3beta (unknown origin)
Inhibition of GSK-3beta (unknown origin) in presence of ATP
Inhibition of human GSK-3beta using peptide substrate after 45 mins in presence of ATP by Kinase-Glo luminescence assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of GST tagged GSK-3beta (unknown origin) incubated for 1 hrs by HTRF assay
Assay data:34 Active, 2 Activity ≤ 1 nM, 34 Activity ≤ 1 µM, 34 Tested
Inhibition of GSK-3beta (unknown origin) assessed as decrease in tau phosphorylation by high content cell based assay
Assay data:32 Active, 29 Activity ≤ 1 µM, 32 Tested
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