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Inhibition of human GFP-tagged SLC40A1 expressed in HEK293 cells assessed as ferroportin internalization by fluorescence microscopic analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Binding affinity to recombinant human C-terminal nano luciferase-tagged SLC40A1 expressed in human T-REx-293 cells assessed as internalization of compound-ferroportin conjugate at 0.0001 to 0.01 mg/ml incubated for 20 hrs measured at 549 nanometer by Hoechst staining based cellomics arrayscan imaging analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of recombinant human C-terminal nano luciferase-tagged SLC40A1 expressed in human T-REx-293 cells assessed as ferroportin internalization incubated for 20 hrs measured at 459 nanometer by Hoechst staining based cellomics arrayscan imaging analysis
Inhibition of recombinant human SLC40A1 expressed in human T-REx-293 cells exposed to >4 hrs of doxycycline induction assessed as reduction in ferroportin internalization incubated for 20 hrs by luciferase reporter gene assay relative to control
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of recombinant human SLC40A1 expressed in human T-REx-293 cells exposed to 4 hrs of doxycycline induction assessed as reduction in ferroportin internalization incubated for 20 hrs by luciferase reporter gene assay relative to control
Inhibition of recombinant human SLC40A1 expressed in human T-REx-293 cells assessed as reduction in ferroportin internalization incubated for 20 hrs by luciferase reporter gene assay
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
Inhibition of recombinant human C-terminal nano luciferase-tagged SLC40A1 expressed in human T-REx-293 cells assessed as decrease in 55Fe-apotransferrin export at 10 uM preincubated for 24 hrs followed by export buffer and compound addition measured after 5 mins by scintillation counting method
Inhibition of ferroportin in human T47D cells assessed as blocking of iron export incubated for 20 hrs using [58]Fe by inductively coupled plasma mass spectrometry
Inhibition of GFP-tagged human ferroportin expressed in HEK293 #354 cells assessed as induction of ferroportin internalization and degradation incubated overnight by FACS assay
Assay data:90 Active, 40 Activity ≤ 1 µM, 107 Tested
Inhibition of GFP-tagged human ferroportin expressed in HEK293 #354 cells assessed as reduction in Fpn-mediated iron efflux incubated overnight by iron regulatory element -driven beta-lactamase reporter gene assay
Assay data:97 Active, 21 Activity ≤ 1 µM, 130 Tested
Binding affinity to C-terminal FLAG-tagged human ferroportin expressed in Pichia pastoris assessed as reduction in inhibition of binding of TMR-hepcidin to Fpn incubated for 90 mins by fluorescence polarization assay
Assay data:167 Active, 105 Activity ≤ 1 µM, 185 Tested
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Human Ferroportin (SLC40 iron transporter)
Antagonist activity at C-terminal halo-tag fused human ferroportin expressed in MDCK cells assessed as inhibition of hepcidin-induced ferroportin internalization after overnight incubation by Draq5 staining-based fluorescence assay
Assay data:156 Active, 43 Activity ≤ 1 µM, 250 Tested
Agonist activity at GFP-fused human FPN transfected in HEK293 cells assessed as protein degradation after 24 hrs by flow cytometric analysis
Assay data:20 Active, 29 Activity ≤ 1 µM, 30 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
Induction of human ferroportin degradation expressed in HEK293 cells after 24 hrs by flow cytometry
Assay data:9 Active, 9 Activity ≤ 1 µM, 9 Tested
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
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