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Prodrug conversion in pH 7.4 PBS buffer assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd release by measuring Km at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under aerobic condition by Lineweaver-Burk plot analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Prodrug conversion in pH 7.4 PBS buffer assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd release by measuring Km at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under hypoxic condition by Lineweaver-Burk plot analysis
Prodrug conversion in pH 7.4 PBS buffer assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd release by measuring Vmax at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under aerobic condition by Lineweaver-Burk plot analysis
Prodrug conversion in pH 7.4 PBS buffer assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd release by measuring Vmax at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under hypoxic condition by Lineweaver-Burk plot analysis
Prodrug conversion assessed as NADPH-cytochrome P450 reductase (unknown origin) mediated F-OH formation by measuring Vmax at 0.93 mM in the presence of beta-NADPH measured after 15 mins under hypoxic condition by Lineweaver-Burk plot analysis
Prodrug activation in pH 7.4 PBS buffer assessed as 3.3 ug/mL NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd formation at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under aerobic condition by reverse phase HPLC
Prodrug activation in pH 7.4 PBS buffer assessed as 3.3 ug/mL NADPH-cytochrome P450 reductase (unknown origin) mediated FdUrd formation at 0.62 mM in the presence of beta-NADPH measured after 2 hrs under hypoxic condition by reverse phase HPLC relative to control
Assay data:2 Tested
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Substrate activity at human CPR expressed in baculovirus expression system assessed as reduction rate by measuring umol of NADPH utilized per umol of enzyme at 10 uM preincubated for 3 mins followed by NADPH addition measured at 2 secs interval for 5 mins by UV-Vis spectroscopy
Assay data:8 Tested
Induction of recombinant human CPR-mediated superoxide generation assessed as one-electron reduction of compound by measuring SOD-inhibitable reduction of succinoylated cytochrome c per 2 mU enzyme at 100 umol/ml by spectrophotometric analysis (Rvb = 0.2 +/- 0.1 micromol/L/min)
Assay data:7 Tested
Prodrug activation assessed as supersomal P450 oxido-reductase (unknown origin)-mediated CA4 level at 2.5 uM in presence of 0.5% O2 condition after 2 mins by HPLC method
Substrate activity at recombinant human CPR expressed in baculovirus infected insect cells assessed as compound reduction rate by measuring NADPH consumption per umol of enzyme preincubated for 3 mins followed by NADPH addition measured for 5 mins at 2 sec time interval by UV-Visible spectroscopic method
Assay data:23 Tested
Substrate activity at CPR in human L02 cells assessed as CPR-mediated one-electron reduction of compound by measuring cell growth inhibition treated for 24 hrs measured after 72 hrs by MTT assay
Assay data:2 Active, 11 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Substrate activity at CPR in human L02 cells assessed as CPR-mediated one-electron reduction of compound by measuring cell growth inhibition at 20 to 100 uM treated for >8 hrs measured after 72 hrs by MTT assay
Assay data:1 Active, 1 Tested
Substrate activity at CPR in human L02 cells assessed as CPR-mediated one-electron reduction of compound by measuring ROS production after 1 to 3 hrs by DCFH-DA staining based flow cytometry
Substrate activity at CPR in human L02 cells assessed as CPR-mediated one-electron reduction of compound by measuring ROS production at 10 uM after 1 to 3 hrs by DCFH-DA staining based flow cytometry
Activity at human recombinant CPR assessed as superoxide generation at 100 umol/ml by spectrophotometric assay
Assay data:7 Active, 7 Tested
Activity of NADPH:cytochrome P-450R (unknown origin) assessed as O2 uptake at 5 to 15 uM by polarographic analysis in the presence of 100 U/ml catalase and 150 to 200 uM of NADPH
Assay data:13 Active, 13 Tested
Activity of NADPH:cytochrome P-450R (unknown origin) assessed as NADPH oxidation by spectrophotometric analysis
Assay data:13 Tested
Activity of NADPH:cytochrome P-450R (unknown origin) assessed as O2 uptake at 5 to 15 uM by polarographic analysis in the presence of 150 ug/ml superoxide dismutase and 150 to 200 uM of NADPH
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