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RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Binding affinity to human recombinant UbcH5c assessed as dissociation rate constant by SPR analysis
Assay data:4 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to human recombinant UbcH5c assessed as association rate constant by SPR analysis
Binding affinity to trypsin digested recombinant UbcH5c (unknown origin) assessed as covalent adduct formation with Cys111 residues after 1 hr by LC/MS/MS analysis
Assay data:1 Tested
Binding affinity to trypsin digested recombinant UbcH5c (unknown origin) assessed as covalent adduct formation with Cys85 residues after 1 hr by LC/MS/MS analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to human recombinant UbcH5c by SPR analysis
Assay data:5 Active, 2 Activity ≤ 1 µM, 5 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of UbcH5c (unknown origin) preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Assay data:5 Tested
Inhibition of UbcH5c (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Inhibition of UbcH5c (unknown origin) at 1 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Assay data:6 Tested
Inhibition of UbcH5c (unknown origin) at 5 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Assay data:17 Active, 52 Tested
Inhibition of UbcH5c (unknown origin) at 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis
Assay data:11 Active, 15 Tested
Binding affinity to UbcH5c in human HeLa cells up to 40 uM after 40 mins by Azide-Fluor 488 dye-based fluorescence imaging assay
Binding affinity to UbcH5c in human HeLa cells up to 40 uM after 40 mins by Western blot analysis
Inhibition of UbcH5c in human HeLa cells assessed as TNF-alpha induced linear polyubiquitination of NEMO preincubated for 2 hrs followed by TNF-alpha addition for 5 mins by Western blot analysis
Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced MMP-3 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis
Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced MCP-1 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis
Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced IL-1 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis
Inhibition of UbcH5c (unknown origin) expressed in human 293T cells assessed as decrease in TNF-alpha-mediated NF-kB activation at 5 uM preincubated for 2 hrs followed by TNF-alpha stimulation for 8 hrs by luciferase reporter gene assay relative to control
Assay data:14 Tested
Inhibition of UbcH5c (unknown origin) assessed as RNF4 ubiquitination at 2.5 mM after 15 mins in presence of fluorescently-labeled Ub/E2 by fluorescence assay
Assay data:3 Tested
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
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