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Binding affinity to VWF in human A549 cells lysates grown on SILAC media at 10 uM incubated for 1 hr by LC-MS/MS analysis relative to untreated control
Assay data:1 Tested
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Inhibition of GPIbalpha-vWF binding assessed as constant shear stress at 120 dynes/cm'2-induced platelet agglutination at 10 uM preincubated for 5 mins by cone and plate rheometer analysis relative to control using female platelet rich plasma (Rvb = 100%)
Assay data:2 Tested
Inhibition of GPIbalpha-vWF binding assessed as constant shear stress at 120 dynes/cm'2-induced platelet agglutination at 10 uM preincubated for 5 mins by cone and plate rheometer analysis relative to control using male platelet rich plasma (Rvb = 100%)
Inhibition of GPIbalpha-vWF binding assessed as inhibition of ristocetin-induced platelet agglutination at 100 uM after 2 mins by static agglutination assay using washed platelets from female
Inhibition of GPIbalpha-vWF binding assessed as inhibition of ristocetin-induced platelet agglutination at 100 uM after 2 mins by static agglutination assay using washed platelets from male
Inhibition of GPIbalpha-vWF binding assessed as ristocetin-induced platelet agglutination at 100 uM after 2 mins by static agglutination assay using washed platelets from female (Rvb = 61.7%)
Assay data:3 Tested
Inhibition of GPIbalpha-vWF binding assessed as ristocetin-induced platelet agglutination at 100 uM after 2 mins by static agglutination assay using washed platelets from male (Rvb = 58%)
Inhibition of GPIbalpha-human vWF binding assessed as platelet rolling velocity at 100 uM preincubated for 5 mins by parallel plate flow assay with real time imaging microscopy (Rvb = 7.1 um/sec)
Inhibition of GPIbalpha-vWF binding assessed as ristocetin-induced platelet agglutination at 100 uM after 2 mins by static agglutination assay using platelet rich plasma (Rvb = 76.3%)
Inhibition of GPIbalpha-vWF binding assessed as ristocetin-induced platelet agglutination at 10 uM after 2 mins by static agglutination assay using platelet rich plasma (Rvb = 76.3%)
Inhibition of GPIbalpha-vWF binding assessed as ristocetin-induced platelet agglutination at 1 uM after 2 mins by static agglutination assay using platelet rich plasma (Rvb = 76.3%)
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