BioAssay by Target (List) for Gene (Select 81788) - PubChem BioAssay - NCBI

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Select item 19142091.

Selectivity interaction (Kinase panel (ProQinase, FlashPlate?-based radiometric assay, 10 M ATP)) EUB0000006b NUAK2

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1914209

Summary Related BioAssays by Target

Select item 19139402.

Selectivity interaction (KinomeScan (DiscoverX)) EUB0000702a NUAK2

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1913940

Summary Related BioAssays by Target

Select item 19130793.

Affinity Biochemical interaction (Radiometric kinase assay) EUB0000627a NUAK2

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested

AID:: 1913079

Summary Compounds, Active Compounds, activity ≤ 1 µM Related BioAssays by Target

Select item 19126674.

Affinity Phenotypic Cellular interaction (Western Blot (inhibition of phosphorylation of MYPT1 (myosin phosphate-targeting subunit 1) at Ser445 in HEK293 cells)) EUB0000627a NUAK2

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1912667

Summary Related BioAssays by Target

Select item 19005825.

Inhibition of NUAK2 (unknown origin) at 0.1 uM relative to control

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1900582

Summary PubMed Citation Related BioAssays by Target

Select item 19005556.

Inhibition of NUAK2 (unknown origin) at 1 uM relative to control

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1900555

Summary PubMed Citation Related BioAssays by Target

Select item 18950937.

Inhibition of NUAK2 (unknown origin) at 0.5 uM

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1895093

Summary PubMed Citation Related BioAssays by Target

Select item 18455918.

Binding affinity to NUAK2 (unknown origin) assessed as dissociation constant

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested

AID:: 1845591

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 18455899.

Inhibition of NUAK2 (unknown origin) at 1 uM relative to control

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1845589

Summary PubMed Citation Related BioAssays by Target

Select item 184557810.

Inhibition of N-terminal GST-tagged human NUAK2 expressed in Escherichia coli DH5alpha incubated for 30 mins in presence of gamma-[32P]ATP by cerenkov counting analysis

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested

AID:: 1845578

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 184556411.

Inhibition of NUAK2 (unknown origin)

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:3 Active, 2 Activity ≤ 1 µM, 3 Tested

AID:: 1845564

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 183566512.

SNARK DiscoveRx kinase panel

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1835665

Summary Related BioAssays by Target

Select item 183508113.

NUAK2(h) Kinase panel

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1835081

Summary Related BioAssays by Target

Select item 183432514.

Inhibition of SNARK (unknown origin) at 1 uM

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1834325

Summary PubMed Citation Related BioAssays by Target

Select item 182379915.

Inhibition of full length recombinant human NUAK2 using KKKVSRSGLYRSPSMPENLNRPR as substrate incubated for 40 mins in presence of [gamma-33P-ATP] by radiometric scintillation assay

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested

AID:: 1823799

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 181204216.

Binding affinity to wild-type human partial length SNARK (P22 to A333 residues) expressed in mammalian expression system assessed as residual binding level at 100 nM by Kinomescan method relative to control

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Tested

AID:: 1812042

Summary PubMed Citation Related BioAssays by Target

Select item 179434217.

KinomeScan assay: inhibition of SNARK

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Active, 108 Tested

AID:: 1794342

Summary Compounds, Active Related BioAssays by Target

Select item 179376018.

NuaK2(NUA2LHY1) Takeda global kinase panel

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Activity ≤ 1 µM, 1 Tested

AID:: 1793760

Summary Compounds, activity ≤ 1 µM Related BioAssays by Target

Select item 179347319.

NuaK2(NUA2LHY1) Takeda global kinase panel

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Activity ≤ 1 µM, 1 Tested

AID:: 1793473

Summary Compounds, activity ≤ 1 µM Related BioAssays by Target

Select item 179317920.

NuaK2(NUA2LHY1) Takeda global kinase panel

Source:: ChEMBL

Protein Target:: NUAK family SNF1-like kinase 2

Assay data:1 Activity ≤ 1 µM, 1 Tested

AID:: 1793179

Summary Compounds, activity ≤ 1 µM Related BioAssays by Target

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Target

Proteins (1)
BioAssays with protein sequences specified as targets
RNAi Target, Active (8)
BioAssays with gene sequences specified as RNAi targets and the activity is active

BioActivity

Active Compounds (19)
BioAssays with compounds reported as biologically active
Activity (IC50, etc) ≤ 1 nM (4)
BioAssays with the active concentration of tested reagents less than 1 nanomolar
Activity (IC50, etc) ≤ 1 µM (18)
BioAssays with the active concentration of tested reagents less than 1 micromolar

Experiment Type

Summary (1)
BioAssays summarizing information from multiple assays for validated chemical probes or small molecule leads
Confirmatory (24)
Low-throughput BioAssays where the activity outcome is based on a dose-response relationship with multiple tested concentrations
Primary Screening (40)
Primary high-throughput BioAssays where the activity outcome is based on percentage inhibition from a single dose
Chemical Screens (120)
BioAssays reporting BioActivity of chemical reagents
RNAi Screens (44)
BioAssays reporting BioActivity of RNAi reagents

Assay Project (1)
BioAssays organized into assay projects based on linkage to Summary assays. Click an project below to retrieve the bioassay list for the project. Click 'All Assay Projects' to retrieve all assay projects represented by Summary assays.

Depositor Category

Literature Extraction (97)
BioAssays extracted from the literature
NIH MLP (1)
BioAssays deposited by the NIH Molecular Libraries Program
Other (65)
BioAssays deposited by other experimentalists

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