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ATPase enzyme assay from Article 10.1021/acschembio.7b00041: "Discovery and Characterization of a Eukaryotic Initiation Factor 4A-3-Selective Inhibitor That Suppresses Nonsense-Mediated mRNA Decay."
Assay data:2 Active, 1 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay
Assay data:17 Active, 13 Activity ≤ 1 µM, 17 Tested
Inhibition of hippuristanol binding to full-length human recombinant His6-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) by surface plasmon resonance assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to full-length human recombinant His6-tagged eIF4A3 ATP binding site expressed in Escherichia coli BL21(DE3) in presence of 4-chloro-7-fluoro-6-isopropyl-1H-indole-2-carboxylic acid by surface plasmon resonance assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to full-length human recombinant His6-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as dissociation half life by surface plasmon resonance assay
Assay data:3 Tested
Binding affinity to full-length human recombinant His6-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as dissociation rate constant by surface plasmon resonance assay
Binding affinity to full-length human recombinant His6-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as association rate constant by surface plasmon resonance assay
Binding affinity to full-length human recombinant His6-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) by surface plasmon resonance assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 4 Tested
Inhibition of eIF4A3 (unknown origin) expressed in HEK293T cells assessed as NMD suppression at 3 uM after 6 hrs by luciferase reporter gene assay relative to control
Assay data:5 Active, 7 Tested
Inhibition of eIF4A3 (unknown origin) expressed in HEK293T cells assessed as NMD suppression at 10 uM after 6 hrs by luciferase reporter gene assay relative to control
Assay data:7 Tested
Inhibition of MLN51-induced full-length human recombinant His6-tagged eIF4A3 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay
Assay data:21 Active, 11 Activity ≤ 1 µM, 37 Tested
Assay data:4 Tested
Assay data:5 Tested
Inhibition of full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 helicase activity expressed in Escherichia coli BL21(DE3) assessed as reduction in RNA unwinding up to 300 uM using (5'-(FITC)-GCUUUACGGUGC-3')/(5'-GGGGAGAAAAACAAAACAAAACUAGCACCGUAAAGCACGC-3') RNA as substrate in presence of ATP/MLN51 after 30 mins by gel electrophoresis method
Assay data:1 Active, 2 Tested
Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as change in entropy by isothermal titration calorimetric method
Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as change in Gibbs free energy by isothermal titration calorimetric method
Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as dissociation half-life administered for 60 to 120 secs by surface plasmon resonance biosensing assay
Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as dissociation rate constant administered for 60 to 120 secs by surface plasmon resonance biosensing assay
Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as association rate constant administered for 60 to 120 secs by surface plasmon resonance biosensing assay
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