Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Binding affinity to hypoxia induced HIF-1 alpha/P300 CH1 domain (323 to 423 residues expressed in Escherichia coli (DE3)/CBP (unknown origin) interaction assessed as dissociation constant
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of hypoxia-induced HIF1alpha/P300 interaction (unknown origin) incubated for 30 mins for fluorescence anisotropy competition assay
Induction of HIF-1alpha stabilization in human Hep3B cells assessed as HIF-1alpha level at 16 to 1000 nM incubated for 24 hrs by immunoblot analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Induction of HIF-1alpha stabilization in human Hep3B cells assessed as HIF-1alpha level at 1 to 100 uM incubated for 24 hrs by immunoblot analysis
Assay data:3 Tested
Stabilization of HIF-1 alpha in human HeLa cells at 100 uM incubated for 24 hrs by Western blot analysis
Assay data:9 Tested
Inhibition of HIF-1alpha (unknown origin) transcriptional activity expressed in HEK293T cells co-transfected with renilla and HRE-firelfly luciferase incubated for 24 hrs by dual luciferase reporter gene assay
Assay data:22 Active, 15 Activity ≤ 1 µM, 22 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of HA-tagged CTAD (786 to 826 residues) HIF1alpha/Flag-tagged P300 CH1 domain (323 to 423 residues)/CBP (unknown origin) interaction expressed in HEK293T cells measured after 24 hrs by co-iimunoprecipitation assay
Inhibition of hypoxia-induced HIF1alpha/P300-CBP interaction in human HeLa cells at 10 uM measured after 24 hrs by co-immunoprecipiation assay
Inhibition of hypoxia-induced HIF1alpha/P300-CBP interaction mediated gene transcriptional activity in human HeLa cells co-transfected with luciferase reporter plasmid containing HRE and pRL-CMV vector at 25 uM incubated for 24 hrs under hypoxic condition by HRE-dependent dual luciferase reporter gene assay
Inhibition of HIF-1alpha in mouse C2C12 cells transfected with HRE-driven firefly luciferase gene assessed as fold change in transcriptional activity at 300 uM pretreated with compound for 12 hr followed by hypoxic condition for 24 hrs by dual luciferase reporter gene assay
Assay data:1 Active, 12 Tested
Inhibition of HIF-1alpha in mouse C2C12 cells transfected with PDGF-B driven firefly luciferase gene assessed as transcriptional activity pretreated with compound for 12 hr followed by hypoxic condition for 24 hrs by dual luciferase reporter gene assay
Assay data:9 Active, 10 Tested
HIF-1alpha transactivation assays from US Patent US10919843: "Cannabidiol derivatives as inhibitors of the HIF prolyl hydroxylases activity"
Assay data:9 Active, 9 Tested
SummaryCompounds, ActiveRelated BioAssays by DepositorRelated BioAssays by Target
Enzyme Inhibition Assay from Article 10.1016/j.chembiol.2010.03.008: "Utilization of an in vivo reporter for high throughput identification of branched small molecule regulators of hypoxic adaptation."
Assay data:9 Active, 14 Tested
Stabilization of HIF-1alpha from US Patent US10196346: "Substituted acethydrazide derivative, preparation method and use thereof"
Assay data:23 Active, 11 Activity ≤ 1 µM, 26 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Erythropoietin (EPO) Biological Assay from US Patent US10065928: "Quinolinone compound and use thereof"
Assay data:35 Active, 42 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of hypoxia-induced HIF1alpha transcriptional activity in human Hep3B cells co-transfected with luciferase reporter plasmid containing six copies of HREs and pRL-CMV vector assessed as reduction in luciferase activity incubated for 24 hrs under hypoxic condition by HRE-dependent dual luciferase reporter gene assay
Assay data:18 Active, 18 Activity ≤ 1 µM, 25 Tested
Inhibition of hypoxia-induced HIF1 alpha transcriptional activity in human HeLa cells transfected with luciferase reporter containing of HRE assessed as reduction in luciferase activity at 1 uM incubated for 1 hrs under normoxic condition followed by incubation under hypoxic condition for 24 hrs by luciferase reporter gene assay relative to control
Inhibition of hypoxia-induced HIF1 alpha transcriptional activity in human HeLa cells assessed as reduction in VEGF mRNA level at 3 uM incubated for 1 hrs under normoxic condition followed by incubation under hypoxic condition for 24 hrs by quantitative RT-PCR analysis
Inhibition of hypoxia-induced HIF1 alpha transcriptional activity in human HeLa cells assessed as reduction in CA9 mRNA level at 3 uM incubated for 1 hrs under normoxic condition followed by incubation under hypoxic condition for 24 hrs by quantitative RT-PCR analysis
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on