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Enzyme Inhibition Assay from Article 10.1016/j.chembiol.2007.12.010: "Targeting a prokaryotic protein in a eukaryotic pathogen: identification of lead compounds against cryptosporidiosis."
Assay data:3 Active, 4 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
IMPDH Type 1 Enzyme Assay from Article 10.1021/jm070568j: "Probing Binding Requirements of Type I and Type II Isoforms of Inosine Monophosphate Dehydrogenase with Adenine-Modified Nicotinamide Adenine Dinucleotide Analogues."
Assay data:14 Active, 1 Activity ≤ 1 nM, 14 Activity ≤ 1 µM, 14 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Binding affinity to IMPDH1 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay
Assay data:1 Active, 1 Tested
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) using IMP as substrate and DTT containing buffer in presence of NAD
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 10 uM assessed as Kcat for substrate using NAD as fixed substrate at 700 uM and IMP as second substrate with varying concentrations of 25 to 800 uM (Rvb = 21.5 /s)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 10 uM assessed as Km for substrate using NAD as fixed substrate at 700 uM and IMP as second substrate with varying concentrations of 25 to 800 uM (Rvb = 257.5 +/- 23 microM)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 10 uM assessed as Vmax for substrate using NAD as fixed substrate at 700 uM and IMP as second substrate with varying concentrations of 25 to 800 uM (Rvb = 1.5 microM/min)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 10 uM assessed as Kcat for substrate using IMP as fixed substrate at 500 uM and NAD as second substrate with varying concentrations of 31.25 to 1300 uM (Rvb = 21.5 /s)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 10 uM assessed as Km for substrate using IMP as fixed substrate at 500 uM and NAD as second substrate with varying concentrations of 31.25 to 1300 uM (Rvb = 257.5 +/- 23 microM)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 10 uM assessed as Vmax for substrate using IMP as fixed substrate at 500 uM and NAD as second substrate with varying concentrations of 31.25 to 1300 uM (Rvb = 1.5 microM/min)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 2.5 uM assessed as Kcat for substrate using NAD as fixed substrate at 700 uM and IMP as second substrate with varying concentrations of 25 to 800 uM (Rvb = 21.5 /s)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 2.5 uM assessed as Km for substrate using NAD as fixed substrate at 700 uM and IMP as second substrate with varying concentrations of 25 to 800 uM (Rvb = 257.5 +/- 23 microM)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 2.5 uM assessed as Vmax for substrate using NAD as fixed substrate at 700 uM and IMP as second substrate with varying concentrations of 25 to 800 uM (Rvb = 1.5 microM/min)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 2.5 uM assessed as Kcat for substrate using IMP as fixed substrate at 500 uM and NAD as second substrate with varying concentrations of 31.25 to 1300 uM (Rvb = 21.5 /s)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 2.5 uM assessed as Km for substrate using IMP as fixed substrate at 500 uM and NAD as second substrate with varying concentrations of 31.25 to 1300 uM (Rvb = 257.5 +/- 23 microM)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 2.5 uM assessed as Vmax for substrate using IMP as fixed substrate at 500 uM and NAD as second substrate with varying concentrations of 31.25 to 1300 uM (Rvb = 1.5 microM/min)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 0.3 uM assessed as Kcat for substrate using NAD as fixed substrate at 700 uM and IMP as second substrate with varying concentrations of 25 to 800 uM (Rvb = 21.5 /s)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 0.3 uM assessed as Km for substrate using NAD as fixed substrate at 700 uM and IMP as second substrate with varying concentrations of 25 to 800 uM (Rvb = 257.5 +/- 23 microM)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 0.3 uM assessed as Vmax for substrate using NAD as fixed substrate at 700 uM and IMP as second substrate with varying concentrations of 25 to 800 uM (Rvb = 1.5 microM/min)
Inhibition of recombinant Cryptococcus neoformans His-tagged IMPDH expressed in Escherichia coli BL21 (DE3) at 0.3 uM assessed as Kcat for substrate using IMP as fixed substrate at 500 uM and NAD as second substrate with varying concentrations of 31.25 to 1300 uM (Rvb = 21.5 /s)
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