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Inhibition of human full length CBS using L-cysteine and L-homocysteine as substrate at 30 to 300 uM preincubated with enzyme for 1 hr followed by substrate, PLP and SAM addition for 2 hrs by AzMC probe based fluorescence analysis
Assay data:6 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to human CBS by PLP fluorescence-based screening assay
Assay data:2 Tested
Inhibition of CBS (unknown origin) by methylene blue method
Assay data:1 Tested
Inhibition of human CBS by methylene blue method
Inhibition of CBS (unknown origin) by AzMC based fluorescence assay
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of wild type human CBS expressed in HEK293T cells assessed as reduction in H2S contents
Reversible inhibition of full-length GST-fused human CBS using cysteine/homocysteine as substrate assessed as decrease in H2S production at 100 uM preincubated for 1 hr followed by ultrafiltration for 5 times
Assay data:11 Active, 11 Tested
Inhibition of full-length GST-fused human CBS using cysteine/homocysteine as substrate assessed as decrease in H2S production preincubated for 3 mins in presence of homocysteine and lead nitrate followed by cysteine addition
Assay data:9 Active, 12 Tested
Inhibition of full-length GST-fused human CBS using methylcysteine as substrate measured for 10 mins by CPM probe-based fluorescence assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human recombinant CBS assessed as reduction in H2S production at 40 uM using AzMC probe by fluorescence based assay
Inhibition of human CBS Y308A mutant assessed as reduction in H2S production using 2 mM S-methylcysteine as substrate preincubated for 3 mins followed by substrate addition
Inhibition of human CBS Y223A mutant assessed as reduction in H2S production using 2 mM S-methylcysteine as substrate preincubated for 3 mins followed by substrate addition
Inhibition of human CBS H203A mutant assessed as reduction in H2S production using 2 mM S-methylcysteine as substrate preincubated for 3 mins followed by substrate addition
Inhibition of human CBS N194A mutant assessed as reduction in H2S production using 2 mM S-methylcysteine as substrate preincubated for 3 mins followed by substrate addition
Inhibition of human CBS T193A mutant assessed as reduction in H2S production using 2 mM S-methylcysteine as substrate preincubated for 3 mins followed by substrate addition
Inhibition of full-length GST-fused human CBS assessed as reduction in H2S production using 2 mM S-methylcysteine as substrate preincubated for 3 mins followed by substrate addition measured in presence of Triton X-100
Non-competitive inhibition of full-length GST-fused human CBS using S-methylcysteine as substrate by Lineweaver-burk plot analysis
Inhibition of full-length GST-fused human CBS assessed as reduction in H2S production using 20 mM S-methylcysteine as substrate preincubated for 3 mins followed by substrate addition
Assay data:2 Active, 2 Tested
Inhibition of full-length GST-fused human CBS assessed as reduction in H2S production using 2 mM S-methylcysteine as substrate preincubated for 3 mins followed by substrate addition
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Competitive inhibition of full-length GST-fused human CBS up to 10 uM using S-methylcysteine as substrate by Lineweaver-burk plot analysis
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