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Binding affinity to recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells assessed as change in entropy by isothermal titration calorimetry
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells assessed as change in enthalpy by isothermal titration calorimetry
Binding affinity to KCTD12 in wild type mouse brain cytoplasmic fraction using resin-bound immobilized compound incubated for overnight by pull-down experiment based immunoblot analysis
Assay data:2 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to KCTD12 in wild type mouse brain membrane using resin-bound immobilized compound incubated for overnight by pull-down experiment based immunoblot analysis
Binding affinity to Myc-tagged full length KCTD12 (unknown origin) expressed in HEK293 cells using resin-bound immobilized compound by pull down analysis
Binding affinity to recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells assessed as increase in thermal stability of the protein at protein:compound ratio of 1:5 by Thermofluor analysis
Assay data:2 Active, 2 Tested
Binding affinity to recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells assessed as high-order oligomer formation by measuring oligomer molecular weight at protein:compound concentration ratio of 1:2 by SEC-MALS analysis (Rvb = 51.1 +/- 2.1 kDa)
Inhibition of interaction of N-terminal Myc tagged KCTD12 (unknown origin)/Myc-tagged human GABAB1b/rat GABAb2 transfected in HEK293 cells at 200 uM preincubated with KCTD12 for 4 hrs followed by GBR cell lysate addition and measured after 16 hrs by co-immunoprecipitation/immunoblot analysis relative to control
Inhibition of interaction of N-terminal Myc tagged KCTD12 (unknown origin)/Myc-tagged human GABAB1b/rat GABAb2 transfected in HEK293 cells at 20 uM preincubated with KCTD12 for 4 hrs followed by GBR cell lysate addition and measured after 16 hrs by co-immunoprecipitation/immunoblot analysis
Potency index, ratio of Kd of SLQLPILHHAYLPSIGGV to Kd of compound for recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells by isothermal titration calorimetry
Assay data:1 Tested
Potency index, ratio of Kd of RRLSLQLPILHHAYLPSI to Kd of compound for recombinant human 6X His-tagged KCTD12 T1 domain(27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells by isothermal titration calorimetry
Binding affinity to recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells assessed as dissociation constant by isothermal titration calorimetry
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Displacement of TAMRA-AAG-SLQLPILHHAYLPSIGGV from recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells by fluorescence polarization assay
Assay data:25 Active, 13 Activity ≤ 1 µM, 31 Tested
Binding affinity to recombinant human 6X His-tagged KCTD12 T1 domain (27 to 131 residues) expressed in Escherichia coli BL21 DE3 cells preincubated for 1 hr followed by probing with HRP-conjugated 6X-His tag antibody for 0.5 hrs by SPOT peptide microarray analysis
Assay data:28 Active, 19 Activity ≤ 1 µM, 60 Tested
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