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Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as alkylation of L-tryptophan by measuring product formation at pH 7.5 in Tris buffer after 16 hrs by RP-HPLC analysis
Assay data:9 Tested
SummaryPubMed CitationRelated BioAssays by Target
Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as ratio of Kcat/Km in presence of L-tryptophan by Michaelis-Menten plot analysis
Assay data:19 Tested
Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as Km in presence of L-tryptophan by Michaelis-Menten plot analysis
Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as Kcat in presence of L-tryptophan by Michaelis-Menten plot analysis
Substrate activity at recombinant N-terminal His6-fusion tagged Aspergillus fumigatus FgaPT2 expressed in Escherichia coli BL21 (DE3) assessed as alkylation of L-tryptophan by measuring product formation at pH 7.5 in Tris buffer after 16 hrs by RP-HPLC analysis relative to control
Assay data:25 Tested
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