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Reversible inhibition of N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster assessed as restoration of enzyme activity at 2 mM using 3-(1-carboxyvinyloxy)benzoic acid as substrate preincubated for 1 hr in presence of SAM, substrate and Ti(3+)citrate followed by compound washout and subsequent addition of SAM, substrate and Ti(3+)citrate measured after 1 hr by HPLC analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster using 3-(1-carboxyvinyloxy)benzoic acid as substrate preincubated for 15 mins in presence of SAM and substrate followed by Ti(3+)citrate addition and measured by discontinuous HPLC analysis
Assay data:1 Active, 2 Tested
Substrate activity at N-terminal His6-tagged Thermus thermophilus HB8 MqnE expressed in Escherichia coli BL21 (DE3) co-expressing [4Fe-4S]2+ cluster assessed as enzyme-mediated 3-(4-((2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl)-2-carboxybutyl)benzoic acid formation
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